We narrowed to 51,685 results for: son

1. pcDNA3-N-Flag-NLRP3 1-90

   Plasmid

   #75137

   Purpose

   Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 1-90

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

   Plasmid

   #70085

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

   Insert

   mutated human serum transferrin (TF Human)
   

   Tags

   Hexa His tag and N-terminal signal peptide, 4 aa …

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…

   Promoter

   SV40

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-EF1a-hSNCA

   Plasmid

   #170445

   Purpose

   Lentiviral vector expressing human SNCA, under control of EF1alpha promoter.

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   human SNCA (SNCA Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEMS2181

   Plasmid

   #158421

   Purpose

   AAV plasmid with Ple32 (CLDN5 MiniPromoter) driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple32 (CLDN5 Human)
   

   Use

   AAV

   Promoter

   Ple32

   Available Since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX SHP-2(NC)-SH2

   Plasmid

   #46499

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-2(NC) (PTPN11 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-222

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5 CMV 3xFG eIF4G1 +MIC

   Plasmid

   #158776

   Purpose

   pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

   Depositor

   Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

   Insert

   eIF4G1 (with microexon)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT/TO-APT2-LaG16-3W

   Plasmid

   #234425

   Purpose

   Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn-Flex-FlpO

   Plasmid

   #191202

   Purpose

   Cre dependent FlpO expression

   Depositor

   Janelia Viral Tools

   Article

   Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

   Insert

   FlpO
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-WT-WLS-HA-IRES-GFP

   Plasmid

   #178061

   Purpose

   Expression of WLS-HA protein (WT)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. LMPd Amt OXCT1

    Plasmid

    #209407

    Purpose

    Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

    Depositor

    Russell Jones

    Article

    Luda et al Immunity. 2023 Sep 12;56(9):2021-2035.e8

    Insert

    Oxct1 shRNA (Oxct1 Mouse)
    

    Use

    Retroviral

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-cT3G-cERP2-MAFF

    Plasmid

    #192917

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of MAFF

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    MAFF (MAFF Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid

    #115694

    Purpose

    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Inserts

    NmeCas9
    Nme-sgRNA
    

    Use

    CRISPR

    Tags

    NLS and NLS, HA

    Expression

    Insect and Mammalian

    Mutation

    None and fusion of repeat/tracrRNA to make a sgRNA

    Promoter

    EF-1 alpha and U6

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE2-P2A-BSD

    Plasmid

    #174819

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-BSD
    

    Tags

    P2A-BSD and SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-BCL2

    Plasmid

    #163611

    Purpose

    Overexpression of human BCL2

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Insert

    BCL2 (BCL2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid

    #199261

    Purpose

    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    U1a promoter

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

    Plasmid

    #169337

    Purpose

    Expression of the LT-Fucci(SCA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(SCA) (GMNN, CDT1 Human)
    

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-

    Plasmid

    #169338

    Purpose

    Expression of the LT-Fucci(CA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(CA) (GMNN, CDT1 Human)
    

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-N-HA-NEK7 K64M

    Plasmid

    #75143

    Purpose

    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lys 64 mutated to Met; kinase inactive and F236L …

    Promoter

    CMV

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCardinal-Farnesyl-5

    Plasmid

    #56159

    Purpose

    Localization: Plasma Membrane, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid

    #99277

    Purpose

    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

    Depositor

    Jordan Miller

    Article

    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

    Inserts

    H2B-mOrange2-IRES-EGFP
    Puro
    

    Use

    Lentiviral

    Tags

    EGFP, H2B, IRES, PGK, and mOrange2

    Promoter

    EF1 alpha-human and PGK-mouse

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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