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pPB-AMBRA1-217*-3xFLAG

Plasmid

#174161

Purpose

PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217

Depositor

Julien Sage

Publication

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Missing aa 217-1208

Promoter

EF1A

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald R346C

Plasmid

#164213

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Publication

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

R346C

Promoter

EF1a

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald R235Q

Plasmid

#164212

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Publication

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

R235Q

Promoter

EF1a

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pETM30-ARC105

Plasmid

#133426

Purpose

Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

Depositor

Gerhard Wagner

Publication

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

PCQAP (MED15 Human)

Expression

Bacterial

Mutation

Contains amino acids 5-78 fused to C-terminal of …

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKF-P14MM2AG5h

Plasmid

#128254

Purpose

Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

Depositor

Gabor Balazsi

Publication

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

rtTA-Advanced::P2A::EGFP

Use

Synthetic Biology; Flp-in expression vector

Expression

Mammalian

Promoter

tight TRE promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ELF4_pet28a

Plasmid

#131661

Purpose

Bacterial expression of ETS gene ELF4

Depositor

Peter Hollenhorst

Publication

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF4 (ELF4 Human)

Tags

6xHis

Expression

Bacterial

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHybr_r2a>mG2b-srt-his

Plasmid

#124804

Purpose

HDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.

Depositor

Ferenc Scheeren, Martijn Verdoes

Publication

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

Murine IgG2b

Use

CRISPR; Hdr template

Tags

Histag (HHHHHH) and Sortag (LPETGG)

Expression

Bacterial and Mammalian

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBT346.6-H3.3-K4M-bGHpolyA

Plasmid

#128743

Purpose

Inducible expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Publication

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

K4M

Promoter

CAG

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des13

Plasmid

#110213

Purpose

Yeast Surface Display of G6des13

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des13

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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PacrAB-rfp + PmutS-yfp

Plasmid

#121442

Purpose

plasmid reporting the expression from the acrAB and mutS promoters

Depositor

Mary Dunlop

Publication

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

PmutS-yfp
PacrAB-rfp

Use

Synthetic Biology; Pbb vector

Expression

Bacterial

Promoter

PacrAB and PmutS

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pg-dsRedXP-HIF-2alpha

Plasmid

#87209

Purpose

N-terminally dsRedXP-tagged human HIF-2a/EPAS

Depositor

Violaine Sée

Publication

Taylor et al Open Biol. 2016 Sep;6(9). pii: 160195.

Insert

HIF-2a (EPAS1 Human)

Tags

dsRedXP

Expression

Mammalian

Mutation

Q868E

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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EphA2 430-976 pcDNA3.1

Plasmid

#102738

Purpose

Expression of c terminal EphA2 in mammalian cells

Depositor

Jin Chen

Publication

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (430-976aa) (Epha2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG389-PC2

Plasmid

#99087

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea prohormone convertase 2 (PC2)

Depositor

Phillip Newmark

Publication

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

prohormone convertase 2 (PC2)

Use

T/a cloning vector for dsrna generation and the g…

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-D661A

Plasmid

#92236

Purpose

Express D661A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Publication

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-D661A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Aspartate 661 to Alanine

Promoter

T7 promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMGF157

Plasmid

#96972

Purpose

ish1-mCherry insertion cassette with Hygro resistance gene

Depositor

Adam Frost, Wesley Sundquist

Publication

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + hygro…

Promoter

N/A

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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LP711

Plasmid

#90180

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1000-1826

Depositor

Lotte Pedersen

Publication

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa residues 1000-1826

Promoter

CMV

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-1

Plasmid

#87334

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) Tacδ

Plasmid

#81177

Purpose

Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

Depositor

Chinten James Lim

Publication

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tacδ

Tags

KLGFFKR

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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