304,818 results

1. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE-TIGHT-EGFP-donor fw copy

   Plasmid

   #22074

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   TETO-eGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE-TIGHT-EGFP-donor fw copy

   Plasmid

   #22074

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   TETO-eGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-SA-2A-NEO-CAG-RTTA3

   Plasmid

   #60431

   Purpose

   neomycin resistance; constitutive promoter driving reverse tet activator

   Depositor

   Paul Gadue

   Article

   Sim et al Methods Mol Biol. 2015 Jan 29.

   Insert

   rtTA3
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-SA-2A-NEO-CAG-RTTA3

   Plasmid

   #60431

   Purpose

   neomycin resistance; constitutive promoter driving reverse tet activator

   Depositor

   Paul Gadue

   Article

   Sim et al Methods Mol Biol. 2015 Jan 29.

   Insert

   rtTA3
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Laconic/pcDNA3.1(-)

   Plasmid

   #44238

   Purpose

   a genetically-encoded sensor for lactate concentration, transport and metabolic flux

   Depositor

   Luis Felipe Barros

   Article

   San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

   Insert

   Laconic (LACtate Optical Nano Indicator from CECs)
   

   Use

   Lactate biosensor

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Laconic/pcDNA3.1(-)

   Plasmid

   #44238

   Purpose

   a genetically-encoded sensor for lactate concentration, transport and metabolic flux

   Depositor

   Luis Felipe Barros

   Article

   San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

   Insert

   Laconic (LACtate Optical Nano Indicator from CECs)
   

   Use

   Lactate biosensor

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV8)

   Viral Prep

   #44361-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Promoter

   Syn

   Tags

   mCherry (Cre-dependent)

   Available Since

   Sept. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pAAV2/1

   Plasmid

   #112862

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Truncated P5

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV2/1

    Plasmid

    #112862

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Truncated P5

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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