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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,461 results for: SUB

Showing: 61 - 80 of 10461 results

  1. pcDNA3-PKAsub-YPet-NES

    Plasmid
    #138203
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    138203-plasmid-map-sequence-id-307404

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  2. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid
    #84634
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsub(TA)DD-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84634-plasmid-map-sequence-id-176198

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  4. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid
    #84631
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    ERKsub(TA)DD-Venus-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Mutated target Thr residue to Ala.
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84631-plasmid-map-sequence-id-175151

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  5. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub(T/A)-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138208-plasmid-map-sequence-id-307411

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  6. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-Kras
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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  7. pET41-FeoB-OL2/4sub

    Plasmid
    #216746
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL2 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216746-plasmid-map-sequence-id-434435

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  8. pET41-FeoB-OL3/4sub

    Plasmid
    #216747
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL3 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216747-plasmid-map-sequence-id-434432

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  9. pET41-FeoB-OL5/4sub

    Plasmid
    #216748
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL5 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216748-plasmid-map-sequence-id-434431

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  11. pcDNA3-PKAsub(T/A)-YPet-NES

    Plasmid
    #138205
    Purpose
    Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138205-plasmid-map-sequence-id-307407

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  12. pcDNA3-PKAsub-YPet-Kras

    Plasmid
    #138206
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-Kras
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138206-plasmid-map-sequence-id-307409

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  13. pcDNA3-PKAsub(T/A)-RLuc8N-NES

    Plasmid
    #138212
    Purpose
    Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-RLuc8N-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138212-plasmid-map-sequence-id-307414

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  18. Human a4 nicotinic subunit crystallization construct

    Plasmid
    #85841
    Purpose
    pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct
    Depositor
    Ryan Hibbs
    Article
    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do
    Insert
    Human a4 nAChR subunit crystallization construct (CHRNA4 Human)
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85841-plasmid-map-sequence-id-171121

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  19. Human b2 nicotinic subunit crystallization construct

    Plasmid
    #85842
    Purpose
    pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct
    Depositor
    Ryan Hibbs
    Article
    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do
    Insert
    Human b2 nAChR subunit crystallization construct (CHRNB2 Human)
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    C-terminal StrepII tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85842-plasmid-map-sequence-id-171117

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Showing: 61 - 80 of 10461 results