We narrowed to 308 results for: eukaryotic cell
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Plasmid#140868PurposeT7 promoter driven synthesis of C-terminal fusion of sfGFP to TetR repressor (TetR-sfGFP)DepositorInsertTetR-sfGFP expressed with T7 promoter
UseSynthetic BiologyTagssfGFPExpressionBacterialPromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-3xFRB
Plasmid#20223DepositorInsert3xFRB (MTOR Human)
TagsHA and VhExpressionMammalianMutationOne copy of FRB has a T2098L mutationAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUb RxCas13d + RxCas13d guide RNA
Plasmid#176303PurposeExpresses RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
TagsHA tagExpressionInsectPromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgGAL4-4
Plasmid#46916PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)DepositorInsertssgGAL4-4
Puromycin resistance and mCherry
UseCRISPR and LentiviralExpressionMammalianPromoterCMV and U6Available SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet3.0
Plasmid#174080PurposeTetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertTet3.0 tRNA synthetase and tRNA
ExpressionBacterialMutationL270G, Y271L, N311G, C313AAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-2xDHFR
Plasmid#20218DepositorAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-RGR-r10
Plasmid#74370PurposeThis plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r10
UseCRISPR and Synthetic BiologyExpressionYeastPromoterADH1Available SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLew100_myc_BirA*
Plasmid#41716DepositorTypeEmpty backboneUseT. brucei expressionPromoterPARPAvailable SinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAF112
Plasmid#135920PurposeAll-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.DepositorTypeEmpty backboneUseLuciferase; TransposonExpressionMammalianAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-iRGR-r11
Plasmid#74369PurposeThis plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r11
UseCRISPR and Synthetic BiologyExpressionYeastPromoterAHD1Available SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hy_pUbi-p63e mCherry SV40
Plasmid#176300PurposeExpresses mCherry mRNA from the Drosophila Ubi-p63e promoterDepositorInsertmCherry
ExpressionInsectPromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA mCherry SV40
Plasmid#176302PurposeExpresses mCherry mRNA from the Drosophila Metallothionein A promoterDepositorInsertmCherry
Tags3xFLAGExpressionInsectPromoterMtnAAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUb dRxCas13d + RxCas13d guide RNA
Plasmid#176304PurposeExpresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
TagsHA tagExpressionInsectMutationR239A, H244A, R858A, H863APromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUb dPspCas13b + PspCas13b guide RNA
Plasmid#176306PurposeExpresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cellsDepositorInsertPspCas13b
TagsHA tagExpressionInsectMutationH133A, H1058APromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETA-At
Plasmid#53288PurposeContains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and an lcyB cDNA of Arabidopsis thaliana. Use with pY2F to produce alpha-carotene in E. coli.DepositorAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4
Plasmid#125148PurposeLentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.DepositorInsertAcrIIA4-P2A-mCherry
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINTphiC31
Plasmid#127518PurposePlasmid encodes H. sapiens codon optimized Integrase phiC31.DepositorInsertIntegrase phiC31 coding sequence codon optimized for H. sapiens expression.
ExpressionMammalianAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINTBxb1
Plasmid#127519PurposePlasmid encodes H. sapiens codon optimized Integrase Bxb1.DepositorInsertIntegrase Bxb1 coding sequence codon optimized for H. sapiens expression.
ExpressionMammalianAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Stu2 1-590
Plasmid#38314DepositorAvailable SinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUb LwaCas13a + LwaCas13a Guide RNA
Plasmid#176307PurposeExpresses LwaCas13a and its associated guide RNA in Drosophila cellsDepositorInsertLwaCas13a
TagsHA tagExpressionInsectMutationE656G, I1060V, D1076G- please see depositor comme…PromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only