We narrowed to 181 results for: gcamp3

1. pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE

   Plasmid

   #104497

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7b
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

   Plasmid

   #107737

   Purpose

   In the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.

   Depositor

   Rylan Larsen

   Insert

   synaptophysin-GCaMP6s, mRuby3
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV Cag Flex H2B Gcamp6f reverse

   Plasmid

   #74155

   Purpose

   Calcium Sensor

   Depositor

   Loren Looger

   Insert

   Gcamp6f
   

   Use

   AAV

   Tags

   H2B

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV Cag Flex H2B Gcamp6s reverse

   Plasmid

   #74157

   Purpose

   Calcium Sensor

   Depositor

   Loren Looger

   Insert

   Gcamp6s
   

   Use

   AAV

   Tags

   H2B

   Expression

   Mutation

   Promoter

   SpeI

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

   Plasmid

   #107736

   Purpose

   In the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.

   Depositor

   Rylan Larsen

   Insert

   synaptophysin-GCaMP6s, mRuby3
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

   Plasmid

   #104495

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, and AAV1

   Insert

   jGCaMP7s
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-GCaMP5G

   Plasmid

   #31788

   Purpose

   a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

   Depositor

   GENIE Project, Douglas Kim, Loren Looger

   Article

   Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

   Insert

   GCaMP5G
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE

   Plasmid

   #105322

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV1

   Insert

   jGCaMP7c variant 1513
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

   Plasmid

   #105323

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7c variant 1513
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pN1 Lck-GCaMP5G

    Plasmid

    #34924

    Purpose

    Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

    Depositor

    Baljit Khakh

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    Lck-GCaMP5G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Promoter

    CMV

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE

    Plasmid

    #104491

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP7s
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV Synapsin Intron H2B Gcamp 6s wpre Pzac2.1

    Plasmid

    #74150

    Purpose

    calcium sensor

    Depositor

    Loren Looger

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    H2B

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV Synapsin Intron H2B Gcamp 6f wpre Pzac2.1

    Plasmid

    #74144

    Purpose

    Calcium sensor

    Depositor

    Loren Looger

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    H2B

    Expression

    Mutation

    Promoter

    synapsin

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-Ef1a-Flex-Axon-GCaMP7b

    Plasmid

    #135419

    Purpose

    Can be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.

    Depositor

    Rylan Larsen

    Insert

    axon-jGCaMP7b
    

    Use

    AAV

    Tags

    6xHis and GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid

    #105715

    Purpose

    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

    Depositor

    Rylan Larsen

    Has Service

    AAV5

    Insert

    synaptophysin-GCaMP6s
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. VV229: GCaMP6f in fck

    Plasmid

    #58514

    Purpose

    Expresses GCaMP6f under the a-CamKII promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    GCaMP6f
    

    Use

    Lentiviral

    Tags

    C-terminal FCYENEV (ER export motif); this was no…

    Expression

    Mammalian

    Mutation

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPD_Pinx-1::G-CaMP3

    Plasmid

    #117422

    Purpose

    For Calcium imaging, expression of G-CaMP3 under the expression of a Pinx-1 promoter (AIB interneurons)

    Depositor

    Shohei Mitani

    Article

    Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

    Insert

    G-CaMP3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    inx-1

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Fast-GCaMP-EF20

    Plasmid

    #52645

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF20
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D362N/D366N mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

19. Fast-GCaMP-EF16

    Plasmid

    #52642

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D431N/D433N/D435N/E442A mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

20. Fast-GCaMP-EF19

    Plasmid

    #52644

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF19
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T328D/T330D mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only