We narrowed to 3,301 results for: golden gate

1. p365_DG

   Plasmid

   #234688

   Purpose

   Golden gate domesticated pET28b with His10-bdNEDD8 and ALFA

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   ALFA and His10-bdNEDD8

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p371_DG

   Plasmid

   #234694

   Purpose

   Golden gate domesticated pET28b with Intein-Chitin binding domain

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   Intein-Chitin binding domain

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p361_DG

   Plasmid

   #234683

   Purpose

   Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p367_DG

   Plasmid

   #234689

   Purpose

   Golden gate domesticated pET28b with His10-bdNEDD8 and 2X Strep tag

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   2X Strep tag and His10-bdNEDD8

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNDGG039

   Plasmid

   #231339

   Purpose

   Terminator; BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNDGG038

   Plasmid

   #231338

   Purpose

   Terminator; BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNDGG037

   Plasmid

   #231337

   Purpose

   Terminator; BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. L0I75_tUBQ10_GG5-GG6

   Plasmid

   #219714

   Purpose

   Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.

   Depositor

   Jennifer Nemhauser

   Insert

   GG5-tUBQ10-GG6
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   WT terminator with added golden gate linkers

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOGG026

   Plasmid

   #113992

   Purpose

   Level 1 golden gate cloning vector for low copy number stable environmental gene expression

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOGG024

    Plasmid

    #113991

    Purpose

    Level 1 golden gate cloning vector for medium copy number free-living expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOGG216

    Plasmid

    #114002

    Purpose

    Level 2 golden gate cloning vector for medium copy number free-living expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOGG042

    Plasmid

    #113998

    Purpose

    Vector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Tetracycline
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOGG009

    Plasmid

    #113983

    Purpose

    Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Gentamicin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOGG008

    Plasmid

    #113982

    Purpose

    Vector construction module. Kanamycin/neomycin resistance module for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Kanamycin/neomycin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

    Plasmid Kit

    #1000000221

    Purpose

    A positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.

    Depositor

    Eric Cantor

    Available since

    June 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

16. L0I89-pMUTE_GG0-1

    Plasmid

    #219708

    Purpose

    Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pMUTE-GG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT promoter with added golden gate linkers

    Promoter

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L0I74-_tUBQ10_GG4-GG6

    Plasmid

    #219713

    Purpose

    Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG4-tUBQ10-GG6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT terminator with added golden gate linkers

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L0I87-SPCHPromoter_GG0-1

    Plasmid

    #219707

    Purpose

    Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pSPCH-GG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT promoter with added golden gate linkers

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L0I84 - GG0-pAHP6-GG1

    Plasmid

    #219706

    Purpose

    Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pAHP6-GG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT promoter with added golden gate linkers

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTALE64G

    Plasmid

    #35188

    Purpose

    Golden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP addition

    Depositor

    Gang Bao

    Insert

    TAL Effector-VP64-T2A-eGFP
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    +63 C Terminus, VP64 Domain, T2A-GFP

    Promoter

    CMV

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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