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Plasmid#234683PurposeGolden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiTDepositorTypeEmpty backboneUseTagsHis6-3C-3XFLAG-SUMO and Thrombin-HiBiTExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only
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p367_DG
Plasmid#234689PurposeGolden gate domesticated pET28b with His10-bdNEDD8 and 2X Strep tagDepositorTypeEmpty backboneUseTags2X Strep tag and His10-bdNEDD8ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG039
Plasmid#231339PurposeTerminator; BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.DepositorInsertBEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG038
Plasmid#231338PurposeTerminator; BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.DepositorInsertBEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG037
Plasmid#231337PurposeTerminator; BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.DepositorInsertBEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
L0I75_tUBQ10_GG5-GG6
Plasmid#219714PurposeLevel 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.DepositorInsertGG5-tUBQ10-GG6
UseSynthetic BiologyTagsExpressionMutationWT terminator with added golden gate linkersPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Yarrowia lipolytica Golden Gate tool kit
Plasmid Kit#1000000167PurposeYarrowia lipolytica Golden Gate tool kit for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.DepositorAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOGG026
Plasmid#113992PurposeLevel 1 golden gate cloning vector for low copy number stable environmental gene expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG024
Plasmid#113991PurposeLevel 1 golden gate cloning vector for medium copy number free-living expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG042
Plasmid#113998PurposeVector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectorsDepositorInsertTetracycline
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG216
Plasmid#114002PurposeLevel 2 golden gate cloning vector for medium copy number free-living expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG009
Plasmid#113983PurposeVector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectorsDepositorInsertGentamicin
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG008
Plasmid#113982PurposeVector construction module. Kanamycin/neomycin resistance module for construction of Golden Gate cloning vectorsDepositorInsertKanamycin/neomycin
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
L0I89-pMUTE_GG0-1
Plasmid#219708PurposeLevel 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pMUTE-GG1
UseSynthetic BiologyTagsExpressionMutationWT promoter with added golden gate linkersPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I74-_tUBQ10_GG4-GG6
Plasmid#219713PurposeLevel 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.DepositorInsertGG4-tUBQ10-GG6
UseSynthetic BiologyTagsExpressionMutationWT terminator with added golden gate linkersPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I87-SPCHPromoter_GG0-1
Plasmid#219707PurposeLevel 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pSPCH-GG1
UseSynthetic BiologyTagsExpressionMutationWT promoter with added golden gate linkersPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I84 - GG0-pAHP6-GG1
Plasmid#219706PurposeLevel 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pAHP6-GG1
UseSynthetic BiologyTagsExpressionMutationWT promoter with added golden gate linkersPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
Golden Gate TALEN and TAL effector kit 2.0
Plasmid Kit#1000000024PurposeUsed to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.DepositorAvailable sinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTALE64G
Plasmid#35188PurposeGolden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP additionDepositorInsertTAL Effector-VP64-T2A-eGFP
UseTALENTagsExpressionMammalianMutation+63 C Terminus, VP64 Domain, T2A-GFPPromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTALE64
Plasmid#35189PurposeGolden Gate Compatible TALE Transcription Factor using VP64 DomainDepositorInsertTranscription Activator Like Effector Transcription Factor
UseTALENTagsExpressionMammalianMutation+63 C Terminus, VP64 DomainPromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only