We narrowed to 6,371 results for: kit

1. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

   Plasmid

   #217340

   Purpose

   crRNA array targeting CD81, B2M, KIT, CD55

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Inserts

   crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
   crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
   crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
   crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD649-HAsp-KIT-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156885

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIT (KIT Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD649-HAsp-KIT-COMP5AP-AviTag-9xHis

   Plasmid

   #157449

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIT (KIT Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

   Plasmid

   #207135

   Purpose

   DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

   Plasmid

   #182682

   Purpose

   Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
   

   Expression

   Mammalian

   Promoter

   NA

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

   Plasmid

   #182685

   Purpose

   Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
   mCherry
   

   Expression

   Mammalian

   Promoter

   CMV and NA

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor

   Plasmid

   #182686

   Purpose

   Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
   

   Expression

   Mammalian

   Promoter

   NA

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD132.49

   Plasmid

   #1724

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pPD138.11

   Plasmid

   #1721

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Tags

   EGFPN, EGFPC, IVSL, myo3-2341, myo3Tsa

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPD157.63

    Plasmid

    #1710

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    vit2tsa, T20G511N, T20G511C, let8583', decoy

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPD158.117

    Plasmid

    #1711

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    unc119tsa, RFPN, RFPivs2, RFPV105A, RFPivs4, RFPS…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPD107.09

    Plasmid

    #1703

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    SupTrp5', SupTrp3', T7p, lacZN, OriF1&g…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPD152.79

    Plasmid

    #1704

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    dpy30Tsa, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, …

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPD187.24

    Plasmid

    #1723

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    pie1tsa, SPGFPN, SPGFPC, pie13', T3p, OriF1&…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPD187.49

    Plasmid

    #1722

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    T7p, SPGFPN, SPGFPC, Sp6p, OriF1>>, OriF1&l…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPD159.88

    Plasmid

    #1707

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    rol6tsa, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, I…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPD95_15

    Plasmid

    #1509

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

18. L754

    Plasmid

    #1686

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    IVSA, rf2128

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPD176.36

    Plasmid

    #1720

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    T7p, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, lacIN…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPD117.20

    Plasmid

    #1712

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    unc37-448, unc37Tsa, gfpN, IVSR, gfpF64L, gfpS65T…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge