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Plasmid#112868PurposeBacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner methodDepositorInsertAPEX2
UseTagsExpressionMutationPromoterAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
APEX2 - Actin in pEGFP
Plasmid#66172PurposeAPEX2 fused to the N-terminus of human beta actinDepositorInsertAPEX2 - human beta Actin (ACTB Synthetic, Human)
UseTagsFlag epitope tag (DYKDDDDK)ExpressionMammalianMutationAPEX2 contains 4 mutations relative to wt soybean…PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-COX4-dAPEX2
Plasmid#117176PurposePlasmid name in publication: pAAV-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertCOX4-dAPEX2
UseAAVTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
APEX2 - Tubulin in pEGFP
Plasmid#66171PurposeAPEX2 fused to the N-terminus of alpha tubulinDepositorInsertAPEX2 - alpha tubulin
UseTagsFlag epitope tag (DYKDDDDK)ExpressionMammalianMutationAPEX2 contains 4 mutations relative to wt soybean…PromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2-GAL9
Plasmid#149520PurposeExpress APEX2-Galectin-9 in mammalian cellsDepositorInsertLGALS9 (LGALS9 Human)
UseTagsAPEX2ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-LACTB-dAPEX2
Plasmid#117179PurposePlasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAVTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO C1-APEX2
Plasmid#129641PurposeExpress ER-localized C1-APEX2 fusion in mammalian cellsDepositorInsertC1(1-29)-APEX2-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xflag-APEX2-NES
Plasmid#164623PurposeExpresses a nuclear exporting signal (NES) tagged with APEX2 and 3xflagDepositorInsertNES
UseTags3xFLAG and APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-PARP1-Apex2–eGFP
Plasmid#211555PurposeA piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.DepositorInsertPARP1 (PARP1 Human)
UsePiggybacTagsApex2-eGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_V5-APEX2-HMOX1
Plasmid#85824PurposeExpressed N-term tagged APEX2 on HMOX1 in mammalian cellDepositorInsertV5-APEX2-HMOX1 (HMOX1 Synthetic, Human)
UseTagsAPEX2 and V5ExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-dAPEX2
Plasmid#117174PurposePeroxidase reporter for multiplexed electron microscopy labelingDepositorInsertdAPEX2
UseAAV and Cre/LoxTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKH159_FLAG-APEX2-YIPF4 WT
Plasmid#214978PurposeFlag-APEX2 tagged YIPF4DepositorInsertFLAG-APEX2-YIPF4 (YIPF4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKH161_FLAG-APEX2-YIPF3 WT
Plasmid#214980PurposeFlag-APEX2 tagged YIPF3DepositorInsertFLAG-APEX2-YIPF3 (YIPF3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Vimentin - APEX2 in pECFP
Plasmid#66170PurposeAPEX2 fused to the C-terminus human vimentinDepositorInserthuman Vimentin - APEX2 (VIM Synthetic, Human)
UseTagsFlag epitope tag (DYKDDDDK)ExpressionMammalianMutationAPEX2 contains 4 mutations relative to wt soybean…PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-APEX2
Plasmid#109420PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_V5-APEX2-Sec61B
Plasmid#83412PurposeExpressed N-term tagged APEX2 on Sec61B in mammalian cellDepositorInsertV5-APEX2-Sec61B (SEC61B Synthetic, Human)
UseTagsAPEX2 and V5ExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRiboI-Sec-APEX2m
Plasmid#176845PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)DepositorInsertpRibo-Sec-APEX2m
UseTagsExpressionBacterialMutationPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2pA2-drl:APEX2-mCherry
Plasmid#188945PurposePlasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoterDepositorInsertdrl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA
UseTol2 destination vector (attr4-r3)TagsExpressionMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis APEX2-eIF4A1
Plasmid#129645PurposeExpress APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteriaDepositorInserteukaryotic translation initiation factor 4A1 (EIF4A1 Human)
UseTags6xHis and APEX2ExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Sec-APEX2m
Plasmid#176844PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)DepositorInsertpRibo-Sec-APEX2m
UseInducibleTagsExpressionBacterialMutationPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only