Skip to main content
Addgene
Showing: 781 - 800 of 876 results

  1. pDule-Tet2.0

    Plasmid
    #85496
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85496-plasmid-map-sequence-id-169865

    Enlarge

  2. TFORF2452

    Plasmid
    #142945
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MLLT10 (MLLT10 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142945-plasmid-map-sequence-id-397303

    Enlarge

  3. TFORF1472

    Plasmid
    #141825
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TOX2 (TOX2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141825-plasmid-map-sequence-id-415041

    Enlarge

  6. TFORF1471

    Plasmid
    #143814
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TOX2 (TOX2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143814-plasmid-map-sequence-id-396279

    Enlarge

  7. TFORF3535

    Plasmid
    #145011
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MLLT10 (MLLT10 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145011-plasmid-map-sequence-id-287147

    Enlarge

  8. TFORF0335

    Plasmid
    #144358
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF91 (ZNF91 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144358-plasmid-map-sequence-id-352989

    Enlarge

  13. His-DNPH1

    Plasmid
    #203224
    Purpose
    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    DNPH1 (DNPH1 Human)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203224-plasmid-map-sequence-id-400075

    Enlarge

  17. pFLAG-WTS-1

    Plasmid
    #66856
    Purpose
    Express FLAG-tagged C.elegans WTS-1 in mammalian cells
    Depositor
    Yutaka Hata
    Article
    Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.
    Insert
    WTS-1 (wts-1 Nematode)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E153G and D871G mutations in WTS1 compared to Gen…
    Promoter
    CMV
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. TFORF3009

    Plasmid
    #144485
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF35 (ZNF35 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144485-plasmid-map-sequence-id-407360

    Enlarge

Showing: 781 - 800 of 876 results