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pYPQ-dAaCas12b-Act3.0

Plasmid

#158413

Purpose

CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-Spastin(87-616)DeltaExon4

Plasmid

#128794

Purpose

Bacteiral expression of Spastin M87 varient without exon 4

Depositor

Chris Hill

Article

Han et al J Biol Chem. 2019 Nov 25. pii: AC119.009

Insert

Spastin (SPAST Human)

Use

`

Tags

GST

Expression

Bacterial

Mutation

residues 87-616 without exon 4.

Promoter

T7

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-bCdc42

Plasmid

#23241

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Cdc42 (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
GFP-pCdc42

Plasmid

#23240

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Cdc42 (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
mCherry-CCHD (p130Cas)

Plasmid

#145133

Purpose

Expresses CCH domain from p130Cas with an mCherry tag

Depositor

Johanna Ivaska

Article

Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

Insert

p130Cas CCH domain (BCAR1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-RAB10-WPRE-UbC-Emerald

Plasmid

#203804

Purpose

Lentiviral vector plasmid expressing human RAB10 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Inserts

RAB10 (RAB10 Human)
Emerald

Use

Lentiviral

Expression

Mammalian

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1MycHis/HIP1/ΔE

Plasmid

#31252

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Tags

his and myc

Expression

Bacterial

Mutation

deleted ENTH domain

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pGEX Supt6h-SH2

Plasmid

#46518

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Supt6h (SUPT6H Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1317-1441

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFlox_N-Mau2_BSD_FKBPF36V_EYFP

Plasmid

#163880

Purpose

Acute Depletion of Mau2-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Mau2 (Mau2 Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HIP1/ΔLD

Plasmid

#31253

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Expression

Bacterial

Mutation

Deltion of nucleotides 767-1335

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3/HIP1/ΔC

Plasmid

#31250

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Expression

Bacterial

Mutation

Deltion of nucleotides 1336-2513 1386bp C to T…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
cmv hTid Short

Plasmid

#13709

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Short (DNAJA3 Human)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1MycHis/HIP1/ΔT

Plasmid

#31251

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Tags

his and myc

Expression

Bacterial

Mutation

deletion of TALIN region

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
cmv hTid Long

Plasmid

#13707

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Long (DNAJA3 Human)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pGL3-CMV-Cdc42 3'UTR

Plasmid

#51389

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

Cdc42 3'UTR (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
Lyn-CFP-GAI(1-172)

Plasmid

#37313

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP and Lyn

Expression

Mammalian

Mutation

contains aa 1-172

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pPFAS-2XStrep

Plasmid

#125682

Purpose

expresses human PFAS protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PFAS (PFAS Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin

Plasmid

#134866

Purpose

Expresses EGFP-tagged mutant of VAMP1

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Vesicle-associated membrane protein 1 (VAMP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 97-118

Promoter

EF1a

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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Lyn-CFP-GAI(1-151)

Plasmid

#37312

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP and Lyn

Expression

Mammalian

Mutation

contains aa 1-151

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pQTEV-COASY

Plasmid

#34816

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

Coenzyme A synthase (COASY Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pJT45_GalL_nCDA1-BE3

Plasmid

#145038

Purpose

Expresses nCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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Dll3ICD-HPC4 pBacPAK8-3

Plasmid

#17334

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Delta-like 3 (Dll3 Mouse)

Tags

HPC4

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pISH-Gnas

Plasmid

#105483

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Gnas (Gnas Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBABEhygro-Flag-ZO-2-1st-PDZ-mut

Plasmid

#27474

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

K38E, F44H, I46E and V48E

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
p2Flag CMV2-ZO-2-delta-N

Plasmid

#27419

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Deletion of coding nucleotides from 1-351. This r…

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pJT79_GalL_nCDA1Δ192-BE3

Plasmid

#145045

Purpose

Expresses nCDA1Δ192-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ192-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-192AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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Psicheck2 Sox4 mut 3'UTR oligo

Plasmid

#26988

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

Sox4 mut UTR oligo (SOX4 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

bp 449-509 of SOX4 3'UTR; mut @ bp 483

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pDSHA-ARL4C-Myc

Plasmid

#67360

Purpose

Mammalian expression of hArl4c with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4C (ARL4C Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL4C

Plasmid

#67330

Purpose

hArl4c with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pJL179

Plasmid

#243688

Purpose

Stably expresses SUPV3L1 CDS, flanked with endogenous 5' and 3'UTRs of ALDH18A1

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

UTRs and CDS of SUPV3L1 (SUPV3L1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-GFP

Plasmid

#243759

Purpose

AAV plasmid expressing GFP under the CAG promoter.

Depositor

Andrew Arrant

Article

Nader et al Sci Rep. 2025 Jul 19;15(1):26217. doi: 1

Insert

Enhanced Green Fluorescent Protein

Use

AAV

Promoter

CAG

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mCherry-Rims2[27-179]-mCherry

Plasmid

#246271

Purpose

Rab8 sensor acceptor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mCherry-Rims2[27-179]-mCherry (Rims2 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Amber-free pNL4-3 FLuc Env-RT-

Plasmid

#238415

Purpose

An amber-free HIV NL4-3 carrying a Firefly luciferase reporter gene, RT and Env impaired.

Depositor

Maolin Lu

Article

Xu et al STAR Protoc. 2025 Jul 26;6(3):103995. do

Insert

Firefly luciferase (LOC116160065 Synthetic)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Alternation of amber stop codons "TAG" …

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1_N300

Plasmid

#228814

Purpose

Expression of human EHMT1 N-terminal 300 aa only

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains aa 1-300 only

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP_EHMT1_Ank+SET

Plasmid

#228815

Purpose

Expression of human EHMT1 with deletion of N-terminal 300 aa

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of N-terminal 300 aa

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJC2-Blast-DHFR-3xFLAG

Plasmid

#233892

Purpose

lentiviral overexpression vector for DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

DHFR (DHFR Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Reckleen_3_multieditdropout

Plasmid

#233458

Purpose

Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4

Use

CRISPR

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP_EHMT1B1-3

Plasmid

#228811

Purpose

Expression of mouse EHMT1 with deletion of aa 1185-1298

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 1185-1298

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP_EHMT1B1-4

Plasmid

#228812

Purpose

Expression of mouse EHMT1 with deletion of aa 809-1298

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 809-1298, See Depositor Comments

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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