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Plasmid#122077PurposeExpressing HA-ATG13[3SD]DepositorInsertATG13
UseTags3HAExpressionYeastMutationSerine 646 to aspartic acid; Serine 649 to aspart…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM002
Plasmid#122936PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML043C (Marked)
UseTagsExpressionYeastMutationYML043C: bp 52-63 AAGTTAAAGTAT to AAATTGAAATACPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM001
Plasmid#122935PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML029W (Marked)
UseTagsExpressionYeastMutationYML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTAPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEC005
Plasmid#122939PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML071C (Marked)
UseTagsExpressionYeastMutationYML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGCPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_207
Plasmid#122938PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML070W (Marked)
UseTagsExpressionYeastMutationYML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTAPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_243
Plasmid#122940PurposeExpression of the marked mRNA under the control of insertional promoters.DepositorInsertYML029W (Marked)
UseTagsExpressionYeastMutationYML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTAPromoterPyml029w-tetO(2)Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM004
Plasmid#122937PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML065W (Marked)
UseTagsExpressionYeastMutationYML065W: bp 52-63 GAGCAGGGAAAT to GAACAAGGTAACPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_228
Plasmid#122933PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYBR025C (Marked)
UseTagsExpressionYeastMutationYBR025C: bp 52-63 GGTAATAACTTG to GGAAACAATTTAPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_229
Plasmid#122934PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYBR026C (Marked)
UseTagsExpressionYeastMutationYBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAGPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13[3SD](315)
Plasmid#122074PurposeExpressing GFP-Atg13[3SD]DepositorInsertATG13
UseTagsGFPExpressionYeastMutationSerine 646 to aspartic acid; Serine 649 to aspart…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13[3SA](315)
Plasmid#122073PurposeExpressing GFP-Atg13[3SA]DepositorInsertATG13
UseTagsGFPExpressionYeastMutationSerine 646 to alanine; Serine 649 to alanine; Ser…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIp128-P30-His-pol30(K127R)
Plasmid#99543PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; Leu2 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationK127RPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH3F-PYK1
Plasmid#85774PurposeN-ICE plasmid pH3F-PYK1DepositorInsertloxP-HphMX-loxP-PYK1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTCW010
Plasmid#83521PurposeAromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha2 geneDepositorInsertpARO9-mfalpha2
UseTagsExpressionYeastMutationPromoterpARO9Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW009
Plasmid#83523PurposeAromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha1 geneDepositorInsertpARO9-mfalpha1
UseTagsExpressionYeastMutationPromoterpARO9Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PHBA01
Plasmid#83535PurposeConstitutive TEF1 promoter mediated expression of the UBiC geneDepositorInsertpTEF1-UBiC-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW005
Plasmid#83524Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 1 geneDepositorInsertpFUS1-malpha1
UseTagsExpressionYeastMutationPromoterpFUS1Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
p414GPD_Hsp82viiB
Plasmid#61709PurposeYeast plasmid with Hsp82 heterodimer construct.DepositorInsertHsp82viiB (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationHeteromeric coiled coil inserted after amino acid…PromoterGPD1Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGLx2-set1-H1017K
Plasmid#53258PurposeGAL driven LexA-tagged SET1-catalytically dead (H1017K)DepositorInsertSET1
UseTagsLexAExpressionYeastMutationH1017K mutation - catalytically deadPromoterGAL1/10Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Tpa1(21-644)
Plasmid#23140DepositorInsertTpa1 (TPA1 Budding Yeast)
UseTagsHisExpressionBacterialMutationdeleted amino acids 1-20PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F48
Plasmid#23072DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 14 to Glutamine, H3 K14…PromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F50
Plasmid#23074DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 14 to Arginine, H3 K14R…PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F54
Plasmid#23078DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 9 to Glutamine, H3 K9Q …PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-rB50
Plasmid#18957DepositorInsertBrevican N-terminus (Bcan Rat)
UseTagsExpressionMammalianMutationBrevican N-terminus, aminoacids 1-395PromoterAvailable sinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/T22I
Plasmid#1110DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with threonine 22 changed to isoleucine; re…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G81S
Plasmid#1111DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glycine 81 changed to serine; regulate…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G313S
Plasmid#1114DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glycine 313 changed to serine; regulat…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/E381K
Plasmid#1115DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glutamic acid 381 changed to lysine; r…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/A587T
Plasmid#1116DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with alanine 587 changed to threonine; regu…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/A41V
Plasmid#1117DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with alanine 41 changed to valine; regulate…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
WT Ecm2
Plasmid#169922PurposeEcm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416DepositorInsertEcm2
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
tubP-FRT>GAL80-6-FRT>
Plasmid#63173Purposeubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassetteDepositorInsert"Flp-Out" GAL80
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dsup pLVX-Puro
Plasmid#180635PurposeExpresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio toleranceDepositorInsertDsup
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGAL
Plasmid#60337PurposeContains full length native galactose promoterDepositorInsertspromoter
yECitrine
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPANI2A2E2-Crimson
Plasmid#193758PurposeExpresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPANI2 synthetic promoter carrying binding sites f…Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
KBB418
Plasmid#185088PurposeSwi6L-GFP with nuclear export signal mutated to alaninesDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 nuclear export signal L to A mutationsPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCSUP35(1-253)
Plasmid#1089DepositorInsertSUP35 (SUP35 Budding Yeast)
UseTagsHisExpressionBacterialMutationamino acids 1 - 253 of SUP35PromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJT1
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNC1124
Plasmid#41560DepositorInsertUra3-GAL1-UBIYdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZJOM151
Plasmid#133643PurposeIntegration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.DepositorInsertp1k-COX4-GFP (COX4 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM153
Plasmid#133642PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.DepositorInsertVph1-2GFP (VPH1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PE_CO
Plasmid#190335PurposePrime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cellsDepositorInsertPE2 with codon-optimized reverse transcriptase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXZX501
Plasmid#169455PurposepRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassetteDepositorInsertgRNA for MATa
UseTagsExpressionYeastMutationPromoterSNR52Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM31
Plasmid#133645PurposeIntegration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.DepositorInsertp1k-TGL3-GFP (TGL3 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBPhsFlp2::PEST
Plasmid#63171PurposeDrosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock PromoterDepositorInserthsFlp2::PEST
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXZX351
Plasmid#169454PurposepRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassetteDepositorInsertgRNA for MATα
UseTagsExpressionYeastMutationPromoterSNR52Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH001
Plasmid#67641PurposeHigh copy Cas9 expression vector with LEU2 marker for yeast transformationDepositorInsertsCas9
Empty
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu5 (R)
Plasmid#98296PurposeEnhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM18
Plasmid#133631PurposeIntegration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.DepositorInsertp1k-NAB2-GFP (NAB2 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCMM4
Plasmid#170359PurposeExpresses a Sec61p-mCherry ER-marker from the CYC1 promoterDepositorInsertPCYC1_SEC61_mCherry_LEU2_TCYC1
UseTagsExpressionYeastMutationPromoterPCYC1Available sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only