We narrowed to 73,150 results for: LAS

1. pCRISPaint-mNeon-F-gB-BlastR [M1G]

   Plasmid

   #171807

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.M1G

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-F-gB-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBlastR-5xUAS-mCherry-GMRWhite

   Plasmid

   #165908

   Purpose

   Blasticidin resistant 5xUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable UAS Response Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBlastR-5xQUAS-mCherry-GMRWhite

   Plasmid

   #165920

   Purpose

   Blasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable QUAS Response Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBlastR-12xLexAOp-sfGFP-MiniWhite

   Plasmid

   #165916

   Purpose

   Blasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable LexAOp Response Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV x EF1a_EKAREN4(TA)-ires-blast

   Plasmid

   #167831

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBlastR-5xQUAS-mCherry-MiniWhite

   Plasmid

   #165923

   Purpose

   Blasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable QUAS Response Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMT-blastR-p2a-v5-dL5

   Plasmid

   #161980

   Purpose

   homologous donor template for endogenous tagging

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   blastR-p2a-v5-dL5-GSlinker
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMT-v5-dL5-p2a-blastR

   Plasmid

   #161981

   Purpose

   homologous donor template for endogenous tagging

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   GSlinker-V5-dL5-P2A-blastR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMY-CD90.2-P2A-BlastDYK

   Plasmid

   #163349

   Purpose

   Retroviral vector to express the surface marker CD90.2 and a DYK-tagged blasticidin resistance marker

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   CD90.2 (Thy1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXS-IRES-BLAST PHGDH

    Plasmid

    #154917

    Purpose

    Expresses human PHGDH

    Depositor

    Richard Possemato

    Article

    Richard et al Richard Possemato Lab plasmids

    Insert

    PHGDH (PHGDH Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. JK305: pMVP/Blast-DEST

    Plasmid

    #121855

    Purpose

    pMVP destination vector, empty expression vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. KO901: pMVP (L5-L4) Blast-P2A

    Plasmid

    #121715

    Purpose

    pMVP L5-L4 entry plasmid, contains Blast-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Blast selection marker linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Blast-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti4 Blast RFP-PNRC1

    Plasmid

    #123297

    Purpose

    Mammalian expression of PNRC1 N-RFP

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    PNRC1 (PNRC1 Human)
    

    Use

    Lentiviral

    Tags

    TurboRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX304 PCSK5 (R511C)-V5 blast

    Plasmid

    #83104

    Purpose

    Lentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    PCSK5 (PCSK5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed Arginine 511 to Cysteine

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

15. pLX304 PCSK5 (T288P)-V5 blast

    Plasmid

    #83101

    Purpose

    Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    PCSK5 (PCSK5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed Threonine 288 to Proline

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pLX304 PCSK5 (A270T)-V5 blast

    Plasmid

    #83102

    Purpose

    Lentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    PCSK5 (PCSK5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed Alanine 270 to Threonine

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

17. pLX304 PCSK5 (H516P)-V5 blast

    Plasmid

    #83103

    Purpose

    Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    PCSK5 (PCSK5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed Histidine 516 to Proline

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pEGFP-CLASP2 512-650 9xS/A

    Plasmid

    #24410

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (512-650) 9xS/A (CLASP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable CLASP2 deletion mutant that c…

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAdEasy-EGFP-CLASP2 340-1362

    Plasmid

    #24522

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (340-1362) (CLASP2 Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CLASP2 deletion mutant that retains MT lattice bi…

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pDRM166 LKB (Luciferase mKate2 Blast)

    Plasmid

    #183502

    Purpose

    Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    mKate2
    BSD
    

    Use

    Lentiviral and Luciferase

    Tags

    P2A linker and SV40 NLS with GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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