We narrowed to 55,277 results for: LIS

1. pETSUMO2_Gillisia-bGSDM

   Plasmid

   #212128

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Gillisia bGSDM
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-hGLIS1GR

   Plasmid

   #59312

   Purpose

   Forced expression of human GLIS1GR

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

   Insert

   GLIS1 (GLIS1 Human)
   

   Use

   Retroviral

   Tags

   cGR - hormone binding domain of glucocorticoid re…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLexA::lis-1

   Plasmid

   #11338

   Depositor

   Guy Caldwell

   Insert

   lis-1 (lis-1 Nematode)
   

   Use

   Tags

   LexA DNA Binding Domain

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pRRG88-follistatin

   Plasmid

   #99066

   Purpose

   dsRNA and riboprobe synthesis for Schmidtea mediterranea follistatin

   Depositor

   Phillip Newmark

   Article

   Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

   Insert

   follistatin in situ probe
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGLIS3.1.0-gDNA

   Plasmid

   #113780

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor GLIS3

   Depositor

   Kevin White

   Insert

   GLIS3 (GLIS3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGLIS3-donor

   Plasmid

   #113812

   Purpose

   CRISPR donor plasmid to tag human transcription factor GLIS3 with GFP

   Depositor

   Kevin White

   Insert

   GLIS3 homology arms flanking EGFP-IRES-Neo cassette (GLIS3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   rs10118031, rs10973702, rs912257

   Promoter

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFS_0346_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1_RC

   Plasmid

   #116958

   Purpose

   Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Bacteroides fragilis strain S14 RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFS_0345_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1

   Plasmid

   #116957

   Purpose

   Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Bacteroides fragilis strain S14 RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CL20_mEGFP_CBFA2T3_GLIS2

   Plasmid

   #205786

   Purpose

   Express mEGFP-tagged fusion protein, CBFA2T3_GLIS2 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   CBFA2T3_GLIS2 (CBFA2T3, GLIS2 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCyCEN_Lisp2mCherry_hsp70_GFP

    Plasmid

    #137169

    Purpose

    Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi

    Depositor

    Clemens Kocken

    Article

    Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103

    Inserts

    mCherry
    Nanoluc
    dihydrofolate reductase
    Green Fluorescent Protein
    

    Use

    Unspecified

    Tags

    T2A

    Expression

    Mutation

    Promoter

    P. cynomolgi hsp70 and P. cynomolgi lisp2

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

11. pOliSpec5'

    Plasmid

    #228551

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 5' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOliSpec3'

    Plasmid

    #228550

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LisDPS

    Plasmid

    #122654

    Purpose

    Expression of LisDPS in bacterial cells

    Depositor

    Trevor Douglas

    Article

    Allen et al Inorg Chem. 2003 Oct 6;42(20):6300-5. do

    Insert

    DPS, listeria
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV-U6+27-Tornado-BroccoliSamSensor#1

    Plasmid

    #124363

    Purpose

    Overexpression of circular RNA (Broccoli-based SAM sensor)

    Depositor

    Samie Jaffrey

    Article

    Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

    Insert

    Tornado-BroccoliSamSensor#1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Linked Free ClLIS1 (M)

    Plasmid

    #182487

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS(M)-LS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac_His-ZZ-TEV-Lis1

    Plasmid

    #132539

    Purpose

    Plasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tag

    Depositor

    Andrew Carter

    Article

    Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

    Insert

    Lis1 (PAFAH1B1 Human)
    

    Use

    Tags

    8x His, Linker-TEV-linker_TEV, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs-GLIS1 (FLJ33315SAAN)

    Plasmid

    #33274

    Purpose

    Retroviral expression of canonical human GLIS1

    Depositor

    Shinya Yamanaka

    Article

    Maekawa et al Nature. 2011 . 474(7350):225-9.

    Insert

    GLIS1, Gateway casette A (GLIS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT-Flag-RELISH

    Plasmid

    #63750

    Purpose

    Expresses drosophila RELISH tagged with a Flag epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    Relish (Rel Fly)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    pMT

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pESC-URA-hPOLISc

    Plasmid

    #60819

    Purpose

    to express human Pol iota recoded to yeast codons in yeast

    Depositor

    Youri Pavlov

    Article

    Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

    Insert

    POLI (POLI Human)
    

    Use

    Tags

    c-myc

    Expression

    Yeast

    Mutation

    optimized to yeast codon usage

    Promoter

    GAL1

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCWXPG-UBI-Glis3M412

    Plasmid

    #114301

    Purpose

    Ubiquitous expression of a constitutively active form of the Glis3 transcription factor

    Depositor

    Patrick Salmon

    Insert

    Glis3 (Glis3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    N-terminal deletion up to Methionine 412

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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