We narrowed to 10,450 results for: Ski

1. pCellFree_G03 BMPR1A

   Plasmid

   #67105

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   BMPR1A (BMPR1A Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCellFree_G03 EHD2

   Plasmid

   #67107

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   EHD2 (EHD2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Mutation

   S320T, E384D, T387A, H388Q, T405V, V407A, H418R, …

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCellFree_G03 GTF2H1

   Plasmid

   #67108

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GTF2H1 (GTF2H1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pRevLCav2FeS

   Plasmid

   #60808

   Purpose

   to disrupt iron sulfur cluster in yeast Rev3

   Depositor

   Youri Pavlov, Tahir Tahirov

   Article

   Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

   Insert

   REV3 (REV3 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   contains 647 C-terminal amino acids and 335 bp of…

   Available Since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pBABEpuro-ARAF_D429A

   Plasmid

   #51080

   Purpose

   mammalian expression of ARAF D429A

   Depositor

   Matthew Meyerson

   Article

   Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

   Insert

   ARAF (ARAF Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   D429A

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK048

   Plasmid

   #75230

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

   Promoter

   T7 and n/a

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSPneogRNA241510+MT

   Plasmid

   #84292

   Purpose

   Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA
   

   Use

   CRISPR; Leishmania donovani

   Promoter

   L. donovani ribosome RNA promoter

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. VEGF binding scFv G6

   Plasmid

   #111716

   Purpose

   Yeast Surface Display of G6 as a reference starting point for design

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6
   

   Tags

   cmyc tag

   Expression

   Yeast

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLdCH

   Plasmid

   #84291

   Purpose

   Express gRNA and Cas9 in Leishmania with Hygromycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Promoter

   L. donovani ribosome RNA promoter

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mfn2-YFP

    Plasmid

    #28010

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

    Insert

    mitofusin 2 (MFN2 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    silent S508S

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-GP-2-KDM4A double Tudor

    Plasmid

    #59699

    Purpose

    Contains histone modification interacting domain (KDM4A double Tudor)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    KDM4A double Tudor (KDM4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102886

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF5/FRT/DEST-3xHA/mGli2

    Plasmid

    #51250

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

    Plasmid

    #112837

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Grb2 (Grb2 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SH2 + SH3 (C-term) domains of Grb2

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102887

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102888

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-HSF1Δ∼PRD

    Plasmid

    #71725

    Purpose

    plasmid expressing mic-his tag phosphorylation-deficient hHSF1

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    phosphorylation-deficient HSF1 (HSF1 Human)
    

    Tags

    mac 6xHIS

    Expression

    Mammalian

    Mutation

    15 phosphorylation sites in RD mutated to Alanine

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-GP-2-ATRX ADD

    Plasmid

    #59698

    Purpose

    Contains histone modification interacting domain (ATRX ADD)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    ATRX ADD (ATRX Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5

    Plasmid

    #112840

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Grb2 (Grb2 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Full length Grb2

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102890

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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