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pTK372

Plasmid

#114687

Purpose

sgRNA targeting NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA sgRNA

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac204-TC-OM45-FKBPx4

Plasmid

#104472

Purpose

Integrating plasmid for overexpression of OM45-FKBPx4.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

OM45p (OM45 Budding Yeast)

Tags

FKBPx4- HA

Expression

Yeast

Promoter

TPI1

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCoofy61

Plasmid

#122013

Purpose

E.coli vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

Depositor

Peggy Stolt-Bergner, Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-eGFP

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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CD4d3+4-blac

Plasmid

#32403

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Type

Empty backbone

Tags

beta-lactamase, pentamerization domain of rat car…

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pTK398

Plasmid

#114714

Purpose

mAID-mClover-3FLAG tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

mAID-mClover-3FLAG

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTK711

Plasmid

#114708

Purpose

Express NuMA(1-1985)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-1985)-RFP-Nano

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac204-TC-OM45-FKBPx2

Plasmid

#104471

Purpose

Integrating plasmid for overexpression of OM45-FKBPx2.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

OM45p (OM45 Budding Yeast)

Tags

FKBPx2- HA

Expression

Yeast

Promoter

TPI1

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTK715

Plasmid

#114698

Purpose

Express NuMA(1-2115)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-2115)-RFP-Nano

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy59

Plasmid

#122011

Purpose

Baculovirus vector for parallel SLIC cloning containing N-terminal TrxA-His6 with PreScission Cleavage site

Depositor

Peggy Stolt-Bergner, Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

TrxA-His6

Expression

Insect

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTK746

Plasmid

#114712

Purpose

Express NuMA(1-2115ΔNLS)5A-3-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-2115ΔNLS)5A-3-RFP-Nano

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pHES833

Plasmid

#90409

Purpose

Expresses YFP under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Synthetic Biology

Expression

Yeast

Available Since

Nov. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pProm11_BCD1-GFP

Plasmid

#80656

Purpose

Promoter11 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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MBP-RGG-LgBit-RGG

Plasmid

#176643

Purpose

LgBit fused to LAF-1 RGG domain with N-terminal MBP tag

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

MBP-RGG-LgBit-RGG

Expression

Bacterial

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL

Plasmid

#139798

Purpose

expresses biosynthetic enzyme 4CL

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

4CL

Expression

Bacterial

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLR-NH

Plasmid

#40862

Depositor

Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

last NH repeat

Available Since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pBALU-ext

Plasmid

#69339

Purpose

It allows to extend the genomic insert in a fosmid.

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

kanamycin gene +pCC1FOS homology

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pProm9_BCD1-GFP

Plasmid

#80655

Purpose

Promoter9 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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TC812

Plasmid

#122621

Purpose

QUAS::CFP

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

CFP

Expression

Worm

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy52

Plasmid

#122006

Purpose

E.coli vector for parallel SLIC cloning containing N-terminal His6-3xAvi with PreScission Cleavage site

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-3xAvi

Expression

Bacterial

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTK713

Plasmid

#114699

Purpose

Express NuMA(1-2115ΔNLS)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-2115ΔNLS)-RFP-Nano

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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