We narrowed to 19,051 results for: Tec

1. SFFV-SMASH-CTERM-GFP

   Plasmid

   #185766

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, SMASh

   Mutation

   none

   Promoter

   SFFV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. B*57:03:01

   Plasmid

   #203260

   Purpose

   Overexpress HLA allele

   Depositor

   Derin Keskin, Catherine Wu

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   B*57:03:01 (HLA-B Human)
   

   Expression

   Mammalian

   Available Since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SFFV-SMASH-NTERM-GFP

   Plasmid

   #185761

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, SMASh

   Mutation

   none

   Promoter

   SFFV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-HLA-cmyc-natT1R2

   Plasmid

   #113946

   Purpose

   mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   residues 22-839

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG

   Plasmid

   #206956

   Purpose

   AAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulins

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   mScarlet-I, MmMT3, QtEnc
   

   Use

   AAV

   Tags

   FLAG

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry

   Plasmid

   #71282

   Purpose

   Zebrafish expression vector for heat-shock inducible expression of APEX2-GBP

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2-GBP
   

   Use

   Zebrafish expression with tol2

   Promoter

   HSP70l

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT2 Beta1b GFP

   Plasmid

   #89893

   Purpose

   Mammalian expression of Beta1b GFP

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

   Insert

   Cacnb1 (Cacnb1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   GFP sequence inserted just before the Stop codon

   Promoter

   adenovirus major late promoter

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT2 Beta1b mCherry

   Plasmid

   #89892

   Purpose

   Mammalian expression of Beta1b mCherry

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

   Insert

   Cacnb1 (Cacnb1 Rat)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   mCherry sequence inserted just before the Stop co…

   Promoter

   adenovirus major late promoter

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. AID-BE4max-P2A-GFP

   Plasmid

   #157948

   Purpose

   Mammalian expression of AID-BE4max-P2A-GFP

   Depositor

   Dali Li

   Article

   Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   AID-BE4max
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PET-eSpCas9-plus-NLS-6xHis

    Plasmid

    #207380

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, R1060A, amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SFFV-AID-NTERM-GFP

    Plasmid

    #185763

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, AID

    Mutation

    none

    Promoter

    SFFV

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-hA3A-BE3-D131Y

    Plasmid

    #113424

    Purpose

    Expresses hA3A-BE3-D131Y in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3-D131Y (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_D131Y

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9 TadCBEa-V106W

    Plasmid

    #193838

    Purpose

    Express TadCBEa-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa V106W-SpCas9 D10A
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. His Puc (aa 1-393)

    Plasmid

    #52295

    Purpose

    Bacterial expression of His tagged Drosophila Puc fragment

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Insert

    Puc (puc Fly)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

15. non-targeting control gRNA (BRDN0001146145)

    Plasmid

    #80198

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_UdgX-APOBEC1-UdgX-nCas9

    Plasmid

    #163555

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAPM-miR30-MAVS-ts1

    Plasmid

    #174274

    Purpose

    MAVS knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MAVS shRNA (MAVS Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-hA3H-BE3

    Plasmid

    #113416

    Purpose

    Expresses hA3H-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3H-BE3 (APOBEC3H Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Circular 200,100 with interspersed loops (FANCC)

    Plasmid

    #180192

    Purpose

    AAV vector carrying a guide RNA targeting the human FANCC mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Circular 200,100 with interspersed loops (CTNNB1)

    Plasmid

    #180193

    Purpose

    AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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