We narrowed to 15,737 results for: NTS;

1. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

   Plasmid

   #171588

   Purpose

   Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   Bxb1 integrase
   iCasp9
   BFP
   rtTA3G
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4_CRE-CMVmin-luc2

   Plasmid

   #194384

   Purpose

   Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   6xCRE-CMVmin-luc2
   

   Expression

   Mammalian

   Promoter

   CMV minimal

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207376

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAL-7xHis-TEV-hexa

   Plasmid

   #222868

   Purpose

   Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

   Depositor

   Nikolaus Loening

   Article

   Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

   Insert

   TEV protease
   

   Tags

   7xHis and MBP

   Expression

   Bacterial

   Mutation

   C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. QQR1 variant

   Plasmid

   #138564

   Purpose

   Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   QQR1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5_EGFP-BRD4-NUT

   Plasmid

   #238254

   Purpose

   For overexpression of EGFP-BRD4-NUT

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EGFP-BRD4-NUT
   

   Expression

   Mammalian

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS578_DD-dSpyCas9-mCherry-APEX2

   Plasmid

   #108570

   Purpose

   Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Insert

   dSpyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   APEX2, Destabilization domains, Flag, and mCherry…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ-Cas9-Act3.0

   Plasmid

   #178954

   Purpose

   It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-dual-CRISPR-U6-H1(pBP43)

   Plasmid

   #218930

   Purpose

   The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   human U6 and H1

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-E-Syt2

    Plasmid

    #66831

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt2 (ESYT2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDIRECT_22C

    Plasmid

    #91135

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDIRECT_26G

    Plasmid

    #91149

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA NIA TLS2

    Plasmid

    #196981

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS2 tags
    

    Expression

    Plant

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_PEmax-2A-MLH1dn-2A-BSD

    Plasmid

    #234072

    Purpose

    For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PEmax-2A-MLH1dn
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF-1α core promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. 0_T7-mScarlet3

    Plasmid

    #225927

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EFS-DIO-GRAB_OT1.0

    Plasmid

    #185387

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-MYRIP

    Plasmid

    #89586

    Purpose

    Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYRIP (Myrip Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX4T-E2-25K

    Plasmid

    #63572

    Purpose

    Expression of human Ubiquitin conjugating enzyme E2-25K

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBE2-25K (UBA7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Isoform 1 (200 resiudes)

    Promoter

    tac

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pIN1 (gpdA-cTerm-mGL-ptrA)

    Plasmid

    #188735

    Purpose

    Expresses mGreenlantern for C-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mGreenlantern PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Promoter

    gpdA

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIN2 (gpdA-nTerm-mGL-ptrA)

    Plasmid

    #188736

    Purpose

    Expresses mGreenlantern for N-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mGreenlantern PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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