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pMDC32-Kar2-CFP-HDEL

Plasmid

#96989

Purpose

Express endoplasmic reticulum (ER) marker in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

Kar2-CFP-HDEL

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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DT037A

Plasmid

#159398

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23101 (strong) promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJK_kanR_proJK1_PAD

Plasmid

#60630

Purpose

Constitutively expresses PAD (phenolic acid decarboxylase)

Depositor

Timothy Whitehead

Article

Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

Insert

Phenolic acid decarboxylase padC (padC )

Expression

Bacterial

Mutation

K146Y

Promoter

proJK1

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-hCDC6p-Venus

Plasmid

#212665

Purpose

Express Venus under CDC6 promoter

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

hCDC6p-Venus

Use

Lentiviral

Promoter

hCDC6p

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTK_Axud1_enh_394_Citrine

Plasmid

#130625

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Axud1_enh_394

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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LV-TRE-p65 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60627

Purpose

Expresses p65 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

p65 mouse MyoD

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2

Plasmid

#25752

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pBAD-luxOFP

Plasmid

#229945

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxOFP

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB2-CYCD3;1

Plasmid

#140750

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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SFTPB Promoter reporter

Plasmid

#88868

Purpose

Surfactant Protein B Gene Promoter Reporter

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

Surfactant protein B gene promoter (SFTPB Human)

Use

Luciferase

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ230-D156R (ttLbCas12a)

Plasmid

#195366

Purpose

A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

LbCas12a-D156R (ttLbCas12a)

Use

CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

Expression

Plant

Promoter

ZmUBI1

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-EGFP

Plasmid

#140877

Purpose

126573: Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EGFP

Use

Gateway entry clone

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-L1T1L-NLuc

Plasmid

#128942

Purpose

Expresses L1-NLuc gene

Depositor

Takeshi Kobayashi

Article

Kanai et al J Virol. 2019 May 8. pii: JVI.00401-19.

Insert

L1 minor core protein

Tags

Nanoluc

Expression

Bacterial

Promoter

T7 promotor

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

Plasmid

#66708

Purpose

expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

Depositor

Charles Gersbach

Insert

S.Pyogenes VP64-dCas9-VP64

Use

Lentiviral and Synthetic Biology

Tags

VP64

Mutation

D10A and H840A

Promoter

hUbC

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-luxKate

Plasmid

#229946

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKate

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-YPT31

Plasmid

#18849

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT31 (YPT31 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pCDF1-mAtf4-HT-puro

Plasmid

#101795

Purpose

Lentiviral vector containing C-terminally Halo-tag tagged mouse ATF4

Depositor

Seiichi Oyadomari

Insert

Activating transcription factor 4 (Atf4 Mouse)

Use

Lentiviral

Tags

Halo tag

Expression

Mammalian

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pJDC181

Plasmid

#60853

Purpose

Expresses click beetle red luciferase

Depositor

Jeffrey Cirillo

Article

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Click beetle red luciferase (optimized for Mycobacteria)

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_3

Plasmid

#60564

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_3

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_4

Plasmid

#60565

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_4

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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