We narrowed to 13,392 results for: mpl

1. TDP-43 _CTD_5M→A_IDR

   Plasmid

   #194264

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to Alanine

   Depositor

   Nicolas Fawzi

   Article

   Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Insert

   TDP-43 (TARDBP Human, Synthetic)
   

   Use

   Tags

   6XHistag

   Expression

   Bacterial

   Mutation

   M307A, M311A, M359A, M405A, M414A mutations in TD…

   Promoter

   T7

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TDP-43 _CTD_5M→L_IDR

   Plasmid

   #194265

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucine

   Depositor

   Nicolas Fawzi

   Article

   Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6XHistag

   Expression

   Bacterial

   Mutation

   M307L, M311L, M359L, M405L, M414L mutations in TD…

   Promoter

   T7

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TDP-43 _CTD_5M→Y_IDR

   Plasmid

   #194266

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosine

   Depositor

   Nicolas Fawzi

   Article

   Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Insert

   TDP-43 (TARDBP Human, Synthetic)
   

   Use

   Tags

   6XHistag

   Expression

   Bacterial

   Mutation

   M307Y, M311Y, M359Y, M405Y, M414Y mutations in TD…

   Promoter

   T7

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TDP-43 _CTD_5A→M_IDR

   Plasmid

   #194267

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionine

   Depositor

   Nicolas Fawzi

   Article

   Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Insert

   TDP-43 (TARDBP Human, Synthetic)
   

   Use

   Tags

   6XHistag

   Expression

   Bacterial

   Mutation

   A297M, A367M, A381M, A388M, A391M mutations in TD…

   Promoter

   T7

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP Cav2.2 D122A pcDNA3

   Plasmid

   #206068

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

   Depositor

   Annette Dolphin

   Article

   Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1BR9-AtWUSSTOP-Δα3

   Plasmid

   #202056

   Purpose

   E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.

   Depositor

   Markita Landry

   Article

   Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

   Insert

   AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
   

   Use

   Tags

   6xHis and GFP11

   Expression

   Bacterial

   Mutation

   third helix of homeodomain deleted (AA82-102)

   Promoter

   T7 Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-π-EB1_IRES-mCherry

   Plasmid

   #197427

   Purpose

   Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GCN4 leucine zipper, LOV2, Zdk1, and mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19-π-EB1_EF1α-mCherry

   Plasmid

   #197429

   Purpose

   Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GCN4 leucine zipper, LOV2, Zdk1, and mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19-π-EB1_EF1α-EGFP

   Plasmid

   #197430

   Purpose

   Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   EGFP, GCN4 leucine zipper, LOV2, and Zdk1

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-Duet-6xHis-TEV-Nsp8(76-198)

    Plasmid

    #201024

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBT9-FTS

    Plasmid

    #198526

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    FTS (AKTIP Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBT9-FHIP-L

    Plasmid

    #198528

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    FHIP-L (FHIP1A Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGADT7-δ

    Plasmid

    #197259

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-3 δ (AP3D1 Human)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Val 549 Ile substitution; silent substitutions in…

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBridge-tyrosinase.σ3A

    Plasmid

    #197513

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-3 σ3A
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    silent substitution in codon 2 of tyrosinase tail…

    Promoter

    ADH1 and MET25

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCIneo-V5-SBP-MBP-HsNot9-V181E-shRNA2res_AG

    Plasmid

    #148829

    Purpose

    Mammalian Expression of HsNot9-V181E-shRNA2res

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    HsNot9-V181E-shRNA2res (CNOT9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pnEA-HsNot10_25-707-HsNot11_257-498-CvH_AF

    Plasmid

    #148731

    Purpose

    Bacterial Expression of HsNot10_25-707-HsNot11_257-498

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    HsNot10_25-707-HsNot11_257-498 (CNOT10 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pnEA-NpG-HsNot9_19-285_AF

    Plasmid

    #148733

    Purpose

    Bacterial Expression of HsNot9_19-285

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    HsNot9_19-285 (CNOT9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pnEA-NpH-HsNot9_19-285-Y134DG141W_AE

    Plasmid

    #148618

    Purpose

    Bacterial Expression of HsNot9_19-285-Y134DG141W

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsNot9_19-285-Y134DG141W (CNOT9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmNot1_1149-1376_AC

    Plasmid

    #148475

    Purpose

    Insect Expression of DmNot1_1149-1376

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1_1149-1376 (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    1 silent mutation compared to the sequence given …

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCIneo-lambdaN-HsNot1iso1_1318-1605-HA-MBP_AD

    Plasmid

    #148503

    Purpose

    Mammalian Expression of HsNot1iso1_1318-1605

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    HsNot1iso1_1318-1605 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge