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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,433 results for: tes

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Showing: 8041 - 8060 of 17433 results

  1. pMO14

    Plasmid
    #235736
    Purpose
    Expression of human VPS34 complex II in mammalian cells
    Depositor
    Roger Williams
    Publication
    Mercer et al EMBO J. 2021 Jul 15;40(14):e105985. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    UVRAG (UVRAG Human)
    EGFP
    BECN1 (BECN1 Human)
    PIK3C3 (PIK3C3 Human)
    PIK3R4 (PIK3R4 Human)
    Tags
    2xStrep-tagII-2xTEV-ZZ
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235736-plasmid-map-sequence-id-472856

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  4. pEGFP_D2A_tdTomato

    Plasmid
    #184045
    Purpose
    Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)
    Depositor
    Angela Pannier
    Publication
    Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Enhanced Green Fluorescent Protein
    tdTomato
    Dual 2A Peptide Sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184045-plasmid-map-sequence-id-363159

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  5. kanMX6-ins6

    Plasmid
    #195043
    Purpose
    pFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Publication
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    KanR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195043-plasmid-map-sequence-id-384247

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  6. natMX6-ins5

    Plasmid
    #195042
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Publication
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    NatR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195042-plasmid-map-sequence-id-384245

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  7. kanMX6-ins2

    Plasmid
    #195039
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Publication
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    KanR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    195039-plasmid-map-sequence-id-384242

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  8. AICSDP-079:DCP1A-mEGFP

    Plasmid
    #193922
    Purpose
    Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193922-plasmid-map-sequence-id-380539

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  9. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

    Plasmid
    #187276
    Purpose
    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain
    Depositor
    Alpha Yap
    Publication
    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.
    Insert
    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    deltaActin binding domain
    Promoter
    U6, CMV
    Available since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    187276-plasmid-map-sequence-id-372976

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  11. GFP-Tollip M240A,F241A

    Plasmid
    #178051
    Purpose
    GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.
    Depositor
    David Tumbarello
    Publication
    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Toll-Interacting Protein (TOLLIP Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Changed Methionine 240 to Alanine and changed Phe…
    Promoter
    CMV
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    178051-plasmid-map-sequence-id-350116

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  12. Flag-M4-AR-S81D

    Plasmid
    #171242
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Asp (AR-S81D)
    Depositor
    Steven Balk
    Publication
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Insert
    3xFlag-tagged human androgen receptor Ser81Asp mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Ser81Asp (AR-S81D)
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171242-plasmid-map-sequence-id-336024

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  13. AICSDP-144:CTCF-mEGFP

    Plasmid
    #168799
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CTCF (CTCF Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    June 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    168799-plasmid-map-sequence-id-334694

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  18. pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI

    Plasmid
    #140202
    Purpose
    CRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology; Proof-of-concept te…
    Mutation
    G174D
    Promoter
    PelcA
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140202-plasmid-map-sequence-id-310118

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  19. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid
    #154280
    Purpose
    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Publication
    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; bact…
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154280-plasmid-map-sequence-id-304840

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  20. pCEP4-intron-BaLgp160

    Plasmid
    #100918
    Purpose
    Mammalian expression plasmid for Env from the BaL HIV-1 isolate
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (BaL) Env with upstream intron
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100918-plasmid-map-sequence-id-189993

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Showing: 8041 - 8060 of 17433 results