We narrowed to 14,666 results for: sta
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Plasmid#225702PurposeLentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mTurquoiseExpressionMammalianMutationK27MPromoterpEF1AAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only
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pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD
Plasmid#225698PurposeLentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationK27MPromoterpEF1AAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-RPS27A-UTR-PP7-WPRE
Plasmid#198339PurposePB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loopsDepositorInsertRPS27A-3'UTR-PP7 stem loops x24
UsePiggybacTagsPP7 stem loopsExpressionMammalianPromoterEf1aAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-Dync1h1motor
Plasmid#191330PurposeExpresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
ExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644Available SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-hfYFP
Plasmid#186520PurposeVisualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)DepositorInsertHyperfolder YFP
TagsCytochrome p450 first 29 animo acids (Oryctolagus…ExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Paragranulin+linker 1
Plasmid#176915Purposeexpress Paragranulin with linker 1 in mammalian cellsDepositorInsertParagranulin + linker 1 (GRN Human)
TagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(T17N)
Plasmid#164902PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domainDepositorInsertRaichuEV-Rac1 (RAC1 Human)
ExpressionMammalianMutationT17N mutation in the Rac1 domain which make the s…Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_N116C-G193C
Plasmid#162580PurposeExpresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectMutationAsparagine 116 was mutated to Cysteine and Glycin…PromoterpolyhedrinAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PacrAB-acrAB-rfp + PmutS-mutS-yfp
Plasmid#121443Purposeplasmid expressing translational fusions of AcrAB and MutSDepositorInsertsPmutS-mutS-yfp
PacrAB-acrAB-rfp
UseSynthetic Biology; Pbb vectorExpressionBacterialPromoterPacrAB and PmutSAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-EGFP-TEV
Plasmid#105810PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsEGFP-TEVExpressionMammalianAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRG43-sFRP1
Plasmid#99071PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea sFRP1 (secreted frizzled related protein 1)DepositorInsertsFRP1 (secreted frizzled related protein 1) in situ probe
UseT/a cloning vector for dsrna generation and the g…Available SinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY GBRPL1
Plasmid#89294PurposeFor expression of the mammalian Atg8 protein GABARAPL1 without a tagDepositorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY GBRPL2
Plasmid#89295PurposeFor expression of the mammalian Atg8 protein GABARAPL2 without a tagDepositorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-DCRD-YFP
Plasmid#87450Purposerapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channelsDepositorInsertDCRD (distal C-terminal regulatory domain) (CACNA1F Human)
Tags6xHis-T7, FKBP, and YFPExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) Tac-delta scr
Plasmid#80019PurposeMammalian expression of Tac-delta scrDepositorInsertTac (IL2RA Human)
TagsKLRFGFKExpressionMammalianMutationCD25 amino acids 1 to 263 fused to KLRFGFKPromoterCMVAvailable SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75123PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagMutationbromodomain replaced with the first bromodomain o…Available SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
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RAB14 3'UTR D1
Plasmid#61490Purposeluciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deletedDepositorInsertRAB14 3' UTR (RAB14 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains position 837-1637 (numbering relative to…PromoterPGKAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(767-1356)
Plasmid#49826Purposefor transcribing SRA nucl 767-1356 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
ExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCA-ATG-intron-tdT3Myc
Plasmid#36883DepositorInserttdTomato-3Myc
Tags3 Myc tagsExpressionMammalianMutationbeta-globin intron containing a loxP site inserte…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only