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pBCN27-Pmyo-2::GFP::unc-54_3'UTR

Plasmid

#26347

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

Insert

Pmyo-2::GFP::unc-54_3'UTR

Expression

Worm

Available Since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pGL2_167

Plasmid

#199099

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1 ORI plus NGS barcode 13, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_170

Plasmid

#199102

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI plus NGS barcode 16, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBbB19c-GFP

Plasmid

#126139

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-ZIBG2

Plasmid

#169458

Purpose

cell surface localized zinc sensor with ~ 300 nM Kd

Depositor

Huiwang Ai

Article

Chen et al Anal Chem. 2019 Oct 1;91(19):12212-12219

Insert

ZIBG2

Tags

pDisplay backbone elements for export and membran…

Expression

Mammalian

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1 hSynapsin1 FLEX NAPA-N SV40

Plasmid

#92283

Purpose

FRET probe; extracellular synapse targeted mCherry, Cre-dependently expressed

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Neuronal

Use

AAV

Promoter

hSynapsin1

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB6576

Plasmid

#106134

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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PNMT

Plasmid

#64078

Purpose

Expression of human Phenylethanolamine N-methyltransferase

Depositor

Vern Schramm

Article

Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346

Insert

Phenylethanolamine N-methyltransferase (PNMT Synthetic, Human)

Tags

Histidine tag

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only
pX330-NEAT1pr_v2

Plasmid

#97082

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v2

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-mKate2 no-pA

Plasmid

#80828

Purpose

P2A followed by mKate2 without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-mKate2 no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pNR64

Plasmid

#79540

Purpose

Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

TP901
BxbI

Tags

His tag and LAA degradation tag

Expression

Bacterial

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSBDEST.B

Plasmid

#79460

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Blasticidin selection

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAV0661

Plasmid

#133476

Purpose

Expression in fission yeast from promoter pact1, integrated at ade6 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAH25-SceI

Plasmid

#129389

Purpose

Derived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Chloramphenicol acetyltransferase

Expression

Bacterial

Mutation

N/A

Promoter

CAT promoter

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAdEasy mCherry-Tubulin

Plasmid

#26767

Depositor

Torsten Wittmann

Article

Matov et al Nat Methods. 2010 Sep . 7(9):761-8.

Insert

Tubulin (TUBA1B Human)

Use

Adenoviral

Tags

mCherry

Expression

Mammalian

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only
pME-FlEx switch

Plasmid

#82406

Purpose

Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-GCaMP6s

Plasmid

#184712

Purpose

Calcium biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

GCaMP6s

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Gata4-GFP donor

Plasmid

#113115

Purpose

Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata4 donor region including homology arms and eGFP

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBLO1808_arC9_2xNLS_human

Plasmid

#74491

Purpose

Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

arC9

Tags

t2a mCherry

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pOSML-Cox2-GFP

Plasmid

#203711

Purpose

Overexpression of COX2-GFP fusion

Depositor

Timothy Hla

Article

Trifan et al J Biol Chem. 1999 Nov 26;274(48):34141-7

Insert

Cox2-GFP (PTGS2 Human)

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

SV40

Available Since

June 19, 2024

Availability

Academic Institutions and Nonprofits only

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