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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,433 results for: tes

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Showing: 8081 - 8100 of 17433 results

  1. pGBW-m4092686

    Plasmid
    #153895
    Purpose
    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Bacterial
    Mutation
    Nearly native encoding;inactivating mutations in …
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153895-plasmid-map-sequence-id-307318

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  2. pGL3-sgACSL3.C-Cas9-P2A-Puro

    Plasmid
    #129412
    Purpose
    Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 and sgACSL3.C (ACSL3 Human)
    Use
    CRISPR
    Tags
    P2A-Puro
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129412-plasmid-map-sequence-id-254823

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  3. pCEP4-DU422gp140-TM

    Plasmid
    #123251
    Purpose
    Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (DU422) Env
    Tags
    CD5 leader peptide and Linker (with 6his tag) and…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123251-plasmid-map-sequence-id-235207

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  5. pCEP4-gp120(BaL)-CD4

    Plasmid
    #98957
    Purpose
    Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate
    Depositor
    Erik Procko
    Publication
    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (BaL) gp120
    Tags
    CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    98957-plasmid-map-sequence-id-193406

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  6. pSG5-ERRbeta-delta10

    Plasmid
    #52187
    Purpose
    Expresses human ERRbeta-delta10 splice variant in mammalian cells
    Depositor
    Rebecca Riggins
    Publication
    Heckler et al Cell Cycle. 2014 Dec 12:0.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Estrogen-related receptor beta (ESRRB Human)
    Expression
    Mammalian
    Mutation
    wild type sequence; codon optimized to exclude Ec…
    Promoter
    SV40
    Available since
    April 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  11. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

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  12. pPHR-mCherry-hNCL

    Plasmid
    #181886
    Purpose
    Soluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Publication
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PHR-mCherry-hNCL (NCL Mustard Weed, Human)
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    181886-plasmid-map-sequence-id-359619

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  13. Flag-M4-AR-W741C

    Plasmid
    #171247
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor mutant that binds to bicalutamide: AR-Trp741Cys (AR-W741C)
    Depositor
    Steven Balk
    Publication
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFlag-tagged human androgen receptor Trp741Cys mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Trp741Cys (AR-W741C)
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171247-plasmid-map-sequence-id-336010

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  16. pGL3-Basic-Rab18-sfGFP(N) HDR template

    Plasmid
    #129415
    Purpose
    HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB18 HDR template (RAB18 Human)
    Use
    CRISPR and TALEN; Endogenous tagging hdr template
    Tags
    sfGFP
    Expression
    Mammalian
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129415-plasmid-map-sequence-id-254821

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  19. pCEP4-myc-GRM3-VC

    Plasmid
    #98968
    Purpose
    myc-tagged human mGluR3 fused to C-terminus of split Venus
    Depositor
    Erik Procko
    Publication
    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I A-2 alpha…
    Expression
    Mammalian
    Mutation
    Full-length, wildtype
    Promoter
    CMV
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98968-plasmid-map-sequence-id-193388

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  20. pXJ42-p110 CUX1

    Plasmid
    #100814
    Purpose
    Plasmid vector expressing human p110 CUX1 (amino acids 878-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag.
    Depositor
    Alain Nepveu
    Publication
    Truscott et al Mol Cell Biol. 2008 May;28(10):3127-38.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p110 CUX1 (amino acids 878-1505) (CUX1 Human)
    Tags
    HA and Myc
    Expression
    Mammalian
    Mutation
    Contains amino acids 878-1505
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    100814-plasmid-map-sequence-id-205853

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Showing: 8081 - 8100 of 17433 results