We narrowed to 25,526 results for: CHI

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pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Article

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Tags

Gal4 activation domain

Expression

Yeast

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pUE1-TSP-UL32

Plasmid

#162658

Purpose

Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPOM033_P7_3"His5

Plasmid

#216458

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''his5

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM026_P2_pNMT1

Plasmid

#216451

Purpose

Regulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter nmt1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM019_P2_pRPL2102

Plasmid

#216444

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rpl2102

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM015_P2_pADH1

Plasmid

#216440

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter adh1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM013_P2_pENO101

Plasmid

#216438

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter eno101

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-StrepII-TagRFP-CohI

Plasmid

#58710

Purpose

Detection construct to label dockerin type I containing fusion proteins

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

TagRFP-CohI

Tags

StrepII

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AksgRNA

Plasmid

#121955

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b single chimeric gRNA

Expression

Mammalian

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAJM.713

Plasmid

#108514

Purpose

PLuxB-YFP Reporter. OC6 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PLuxB-YFP

Expression

Bacterial

Available Since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGb_PMLlxP_EGFP

Plasmid

#140283

Purpose

a targeting plasmid for EGFP-PML

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

EGFP

Use

Mouse Targeting

Promoter

endogenous

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pOGG041

Plasmid

#113997

Purpose

Level 0 module. Taurine-inducible promoter for Alphaproteobacteria

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Taurine promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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GST-TEVcs-FIP200-MBP

Plasmid

#171410

Purpose

For the purifcation of human ULK1 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

FIP200 (RB1CC1 Synthetic)

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pOGG216

Plasmid

#114002

Purpose

Level 2 golden gate cloning vector for medium copy number free-living expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA

Plasmid

#121954

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p1.2-Hygro

Plasmid

#162741

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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GFP S2TAG/E17TAG

Plasmid

#68298

Purpose

S2TAG/E17TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

GFP S2TAG/E17TAG

Tags

His

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pKS014

Plasmid

#65467

Purpose

pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidase

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

Ntag-beta galactosidase

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

pl-TetO

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
pUC19-U6-AasgRNA3.8

Plasmid

#121958

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA, short version

Expression

Mammalian

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-hSOX2-EGFP-PuroTK

Plasmid

#141026

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

EGFP

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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