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PROM_Ntpsba_chlo

Plasmid

#163950

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

Insert

Nicotiana tabacum psbA promoter

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_Mprbcl_chlo

Plasmid

#163934

Purpose

L0 part - 5'UTR

Depositor

Jim Haseloff

Article

Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

Insert

Marchantia polymorpha rbcL 5'UTR

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_Mppetb_short_chlo

Plasmid

#163933

Purpose

L0 part - 5'UTR

Depositor

Jim Haseloff

Article

Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

Insert

Marchantia polymorpha petB 5'UTR

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEM-Isce-Tol2-Tn5-neo

Plasmid

#176619

Purpose

for generating PCR templates with Tol2 repeats and Isce sites for BAC recombination

Depositor

Nathan Lawson

Article

Shih et al Development. 2021 Nov 9. pii: 273393. do

Insert

I-SceI sites, Tol2 repeats and Tn5-neo cassette

Use

Unspecified

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIC3

Plasmid

#174036

Purpose

In vitro transcription of anti-CRISPR AcrIIC3 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC3

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIC1

Plasmid

#174035

Purpose

In vitro transcription of anti-CRISPR AcrIIC1 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC1

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_MAPRE1-Halo

Plasmid

#175345

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

MAPRE1 (MAPRE1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF1 (WT)

Plasmid

#175414

Purpose

Encodes His-MBP-TEV-ybbR-WT eRF1

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF1 (SUP45 Budding Yeast)

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBN079

Plasmid

#167960

Purpose

express E. coli E69 (O9a:K30) Wzc with Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN072

Plasmid

#167953

Purpose

express E. coli E69 (O9a:K30) Wzc with Y705E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y705E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y705E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN073

Plasmid

#167954

Purpose

express E. coli E69 (O9a:K30) Wzc with Y706E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y706E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y706E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN075

Plasmid

#167956

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN076

Plasmid

#167957

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN077

Plasmid

#167958

Purpose

express E. coli E69 (O9a:K30) Wzc with Y715E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y715E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y715E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN078

Plasmid

#167959

Purpose

express E. coli E69 (O9a:K30) Wzc with Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA43

Plasmid

#172944

Purpose

pQE30 encoding Wzz mutant i191

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i191 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDH010

Plasmid

#157645

Purpose

sMHT-113 fused non-dimerizing GRX2-C37A mutant

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-GRX2_C37A
GRX2_C37A-sMHT(114-230)

Expression

Bacterial

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb

Plasmid

#141067

Purpose

Multisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBO

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Synthetic Biology

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb-EGFP

Plasmid

#118752

Purpose

Multisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEX

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

EGFP

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-ccdb

Plasmid

#118629

Purpose

Multisite gateway destination vector for 5' tagging with mCherry for cell free expression. Parton lab clone FKZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

mCherry

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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