We narrowed to 19,051 results for: Tec;

1. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

   Plasmid

   #113952

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, I132S and S212N su…

   Promoter

   cmv

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-mCherry-mCherry-FLARE-Cameleon

   Plasmid

   #123352

   Purpose

   Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-Cameleon
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sgRNA BRCA1 E638K

   Plasmid

   #139327

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 E638K using base editing
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCEP4-HA-Flag-natT1R2 FS10

   Plasmid

   #113956

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, S212N substitution…

   Promoter

   cmv

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV_EE-NG-nCas9

   Plasmid

   #163542

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   EE-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET32a-Spike-403-505

   Plasmid

   #165095

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
   

   Expression

   Bacterial

   Available Since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pLenti-BE3RA-PGK-Puro

   Plasmid

   #110843

   Purpose

   Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Mutation

   D10A

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBAD mApple

   Plasmid

   #91772

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   monomeric Apple
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   R97K, E152S, A180S, V207T compared to mApple (FP …

   Promoter

   araBAD

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 2xNLS-NLP-cMyc LbaCas12a

   Plasmid

   #182123

   Purpose

   pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   2xNLS-NLP-cMyc LbaCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. A*01:03:01

    Plasmid

    #203293

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*01:03:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. non-targeting control gRNA (BRDN0001147780)

    Plasmid

    #80226

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. WEE1 gRNA (BRDN0001149349)

    Plasmid

    #77367

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human WEE1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    WEE1 (WEE1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PIM1 gRNA (BRDN0001146465)

    Plasmid

    #77174

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIM1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIM1 (PIM1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PIM1 gRNA (BRDN0001146597)

    Plasmid

    #77175

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIM1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIM1 (PIM1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PIM1 gRNA (BRDN0001145078)

    Plasmid

    #77176

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIM1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIM1 (PIM1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs-ms-Klf1

    Plasmid

    #50785

    Purpose

    retroviral expression of mouse Klf1

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Klf1 (Klf1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CNNM3-CBS-YPet

    Plasmid

    #149686

    Purpose

    For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

    Depositor

    Motoyuki Hattori

    Article

    Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

    Insert

    CNNM3-CBS-YPet (CNNM3 Synthetic, Human)
    

    Tags

    8 histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sgRNA BRCA2 V572I

    Plasmid

    #139321

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 V572I using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 V572I using base editing
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. WEE1 gRNA (BRDN0001145570)

    Plasmid

    #77368

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human WEE1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    WEE1 (WEE1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge