We narrowed to 13,276 results for: SHI

1. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

   Plasmid

   #233064

   Purpose

   Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

   Plasmid

   #233063

   Purpose

   Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Threonine 923 to Glycine

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AID-C12*-n'Cas9-BE4max

   Plasmid

   #216730

   Purpose

   Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

   Insert

   AID-C12*-n'Cas9-BE4max (AICDA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac Dual His6-TEV-CUL2, Rbx1

   Plasmid

   #231000

   Purpose

   Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Inserts

   Rbx1 (Rbx1 Mouse)
   CUL2 (Cul2 Mouse)
   

   Use

   Tags

   6x HIS

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-NPR4 (SA binding domain)

   Plasmid

   #226885

   Purpose

   Expresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble form

   Depositor

   Ning Zheng

   Article

   Wang et al Nature. 2020 Oct;586(7828):311-316. doi:

   Insert

   The Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   NPR4 SA-binding core (residues 373-516), with a s…

   Promoter

   T7

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28:LanIV-Chondromyces

   Plasmid

   #225078

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanIV-Chondromyces)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28:LanII-56

   Plasmid

   #225073

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-56)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28:LanI-101

   Plasmid

   #225065

   Purpose

   Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanI-101)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28:LanII-2E

   Plasmid

   #225072

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-2E)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28:LanII-2D

    Plasmid

    #225071

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2D)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AID-C12*-dCas9-BE4max

    Plasmid

    #216731

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-dCas9-BE4max (AICDA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS

    Plasmid

    #194751

    Purpose

    To fuse your protein of interest to the C-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTol2zmiRFP-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196112

    Purpose

    mammalian/zebrafish expression of zmiRFP-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zmiRFP-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTol2zmiRFP2-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196111

    Purpose

    mammalian/zebrafish expression of zmiRFP2-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zmiRFP2-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196109

    Purpose

    mammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zTagRFP658-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTol2zemiRFP2-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196110

    Purpose

    mammalian/zebrafish expression of zemiRFP2-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zemiRFP2-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-Dest-MBP-Nsp1

    Plasmid

    #191483

    Purpose

    Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    MBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKeratin-emiRFP2-N1

    Plasmid

    #178972

    Purpose

    Expresses the fusion protein of Keratin-emiRFP2 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    Keratin-emiRFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmiRFP2-P2A-HO1-N1

    Plasmid

    #178966

    Purpose

    Expresses miRFP2 and HO1 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2-P2A-HO1
    

    Use

    Tags

    no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-miRFP2-IRES-HO1-N1

    Plasmid

    #178967

    Purpose

    Expresses miRFP2 and HO1 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2
    

    Use

    Tags

    HO1 and no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge