We narrowed to 5,007 results for: puc

1. pUCmini-NFKB2-PGK-tdTomato

   Plasmid

   #204021

   Purpose

   Homologous donor template for NFKB2 knockout expressing tdTomato

   Depositor

   Yohei Sato

   Article

   Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

   Insert

   tdTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-T7-hTP53-P0~670-FLuc

   Plasmid

   #208640

   Purpose

   for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.

   Depositor

   Quanfu Ma

   Insert

   TP53, UTR and partial CDS (TP53 Human)
   

   Use

   Luciferase; In vitor transcription template

   Tags

   Firefly Luciferase (F-Luc) and T7 promoter

   Expression

   Mutation

   UTR and partial CDS in-frame with F-Luc

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC-ODN-hsRPL3-CterTwStrep

   Plasmid

   #208644

   Purpose

   for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
   

   Use

   CRISPR; Ssdna donnor preparation

   Tags

   Twin Strep

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-min5UTR-hRluc-A60

   Plasmid

   #182638

   Purpose

   Transcription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

   Depositor

   Stephen Floor

   Article

   Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

   Insert

   Renilla luciferase,
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-RPLP15UTR-hRluc-A60

   Plasmid

   #182639

   Purpose

   Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

   Depositor

   Stephen Floor

   Article

   Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

   Insert

   Renilla luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   The transcription is driven by a T7 promoter, pai…

   Promoter

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-U6-Bs3-sgRNA (BsaI)

   Plasmid

   #188280

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

   Insert

   Bs3-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   U6

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC-TalO8 (Blank-CoTrIP)

   Plasmid

   #178303

   Purpose

   Blank CoTrIP plasmid

   Depositor

   Brian Zid

   Article

   Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

   Type

   Empty backbone

   Use

   Tags

   NA

   Expression

   Yeast

   Mutation

   Promoter

   NA

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-Peft-pHCountdown-unc54

   Plasmid

   #182304

   Purpose

   C. elegans plasmid encoding pH-Countdown with three introns

   Depositor

   Andrew York

   Article

   Seidel et al (2021), doi: 10.5281/zenodo.5810930

   Insert

   pH countdown with 3 introns, with a Peft3 promoter and unc54 terminator
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Peft-3

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19 KGA HA5'-mKO2-3'

   Plasmid

   #110406

   Purpose

   Homology donor for glutaminase KGA isoform knock-in, mKO2

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-attB2-SA(2)-CRY2-mCherry

    Plasmid

    #160441

    Purpose

    To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

    Depositor

    Nicholas Tolwinski

    Article

    Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57-attB2-SA(1)-CRY2-mCherry

    Plasmid

    #160440

    Purpose

    To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.

    Depositor

    Nicholas Tolwinski

    Article

    Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-attB2-SA(0)-CRY2-mCherry

    Plasmid

    #160439

    Purpose

    To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.

    Depositor

    Nicholas Tolwinski

    Article

    Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. puC19 preACT1(del6) short BP-3'SS

    Plasmid

    #111333

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    shorter branch to 3' splice site distance

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. puC19 preACT1(del6) AcAc

    Plasmid

    #111332

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    3' splice site AcAc

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pre-Ubc4 BrG pUC19

    Plasmid

    #111322

    Purpose

    for transcription to Ubc4 pre-mRNA

    Depositor

    Christine Guthrie

    Article

    Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

    Insert

    UBC4 (UBC4 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    intron has branch-site A to G mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57 DYSF, alt exon 1, 17

    Plasmid

    #87893

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1, 17 (DYSF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57 DYSF, alt exon 1a

    Plasmid

    #87902

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1a (DYSF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC57 DYSF, alt exon 1a, 17

    Plasmid

    #87900

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1a, 17 (DYSF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57 DYSF, alt exon 1a, 5a

    Plasmid

    #87898

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1a, 5a (DYSF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC57 DYSF, alt exon 1a, 5a, 17

    Plasmid

    #87896

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1a, 5a, 17 (DYSF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge