We narrowed to 14,138 results for: eng
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Plasmid#172812PurposeLevel 1 module containing P19_P1 FDepositorInsertp35S-5UTMV-CDS1_P19-3U+TERNOS
UseSynthetic BiologyAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-tdTomato90/816
Plasmid#179918PurposeDouble cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.DepositorInserttdTomato sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterU6Available SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF385A
Plasmid#101615PurposeDonor Vector containing ZNF385A transcription factor, part of the Human TFome CollectionDepositorInsertZNF385A (ZNF385A Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP650
Plasmid#135000PurposeVP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoRDepositorInsertpUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
UseLentiviral and Synthetic BiologyTagsDpnI(aa146-254), V5 tag, and VP-64ExpressionMammalianMutationDpnI truncated to aa146-254Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJ203
Plasmid#162678PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)DepositorInsertPutative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
TagsHisExpressionBacterialMutationdeltaF2-A75; codon optimized for expression in E.…PromoterT7/lacAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
Plasmid#159801PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
Plasmid#159802PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCi-hTRAC-sgRNA
Plasmid#154093PurposepQCi backbone with sgRNA targeting human TCR-alpha common chainDepositorInsertsgRNA targeting human TRAC locus
UseCRISPRExpressionMammalianAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-ITAM only-GFP
Plasmid#126656PurposeInducible expression of Draper-ITAM only-GFP mutant in S2 cellsDepositorInsertDraper with Cytoplasmic ITAM residues Tyr all others mutated to Phe
ExpressionInsectMutationCytoplasmic ITAM residues Tyr all others mutated …Available SinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:FRB:nls:VPR
Plasmid#135987PurposeExpresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cellsDepositorInsertFRB domain fused to the VPR activation domain
TagsHisExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S]
Plasmid#133329PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll FYW to S in CTD of full-length TDP43Available SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 ZFAND3-guide4
Plasmid#125516PurposeCRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralExpressionMammalianPromoterEF1a core and U6Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 EGFP-guide2
Plasmid#118167PurposeCRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA2 against the EGFP CDSDepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralExpressionMammalianPromoterEF1a core and U6Available SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-A8REH9
Plasmid#103084PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertA8REH9(Cas9 coding gene from Eubacterium dolichum DSM 3991)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-K1LM04
Plasmid#103119PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertK1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q4A5I2
Plasmid#103125PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ4A5I2(Cas9 coding gene from Mycoplasma synoviae (strain 53))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q5X8T6
Plasmid#103127PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ5X8T6(Cas9 coding gene from Legionella pneumophila (strain Paris))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3ELL7
Plasmid#103099PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3ELL7(Cas9 coding gene from Bifidobacterium bifidum (strain S17))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E7MR72
Plasmid#103105PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE7MR72(Cas9 coding gene from Solobacterium moorei F0204)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-F3QMY7
Plasmid#103107PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertF3QMY7(Cas9 coding gene from Parasutterella excrementihominis YIT 11859)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only