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JAM2_pLENTI-CAG-IRES-GFP

Plasmid

#176997

Purpose

Mammalian lentiviral expression vector encoding JAM2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

JAM2 (JAM2 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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SOD1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176964

Purpose

Mammalian expression vector encoding SOD1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM3B-FLAG

Plasmid

#180146

Purpose

Coding sequence of human FAM3B in pCS2 with C terminal FLAG tag

Depositor

Edward De Robertis

Article

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

FAM3B (FAM3B Human)

Tags

FLAG

Expression

Mammalian

Promoter

SP6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-POLB-KO-g1

Plasmid

#176090

Purpose

Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

POLB sgRNA #1 (POLB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.3 (19-361)

Plasmid

#177846

Purpose

Bacterial Expression of SnRK2.3

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-CD45

Plasmid

#175943

Purpose

knockout mouse CD45

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

CD45 gRNA (Ptprc Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-Gapdh

Plasmid

#175938

Purpose

knockout mouse Gapdh

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Gapdh gRNA (Gapdh Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE

Plasmid

#174133

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE

Plasmid

#174135

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-Nb127D01-mCherry-KDEL

Plasmid

#171575

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-dre4

Plasmid

#170440

Purpose

Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

dre4 (dre4 Fly)

Tags

6xHis and V5

Expression

Bacterial

Mutation

Deleted amino acids 1-20

Promoter

T7

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-NbVHH05-mCherry-KDEL

Plasmid

#171574

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00008

Plasmid

#166724

Purpose

sgRNA cloning backbone

Depositor

Raj Chari

Article

Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-HaloSFPQY527A

Plasmid

#166949

Purpose

Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

Halo

Mutation

SFPQ-Y527A

Promoter

eF1a

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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UbC-Halo-SFPQY527A

Plasmid

#166947

Purpose

Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

Halo

Mutation

SFPQ-Y527A

Promoter

UbC

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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KLC1N343S-Myc

Plasmid

#166963

Purpose

Expresses Myc-tagged KLC1 N343S protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1-N343S (Klc1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

KLC1-N343S

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 CARD G1467C

Plasmid

#164009

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD G1467C

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

G1467C

Available Since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 UPA-CARD E1461R

Plasmid

#164007

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

E1461R

Available Since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 UPA-CARD W1460A

Plasmid

#164006

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

W1460A

Available Since

Feb. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 UPA-CARD M1457A

Plasmid

#164005

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

M1457A

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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