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pDZ416 (24PP7SL loxP-Kan-loxP)

Plasmid

#45163

Depositor

Robert Singer, Daniel Zenklusen

Article

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

24xPP7 Stem Loop Cassette

Use

Cre/Lox

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pmEGFP_a_tubulin_IRES_puro2b

Plasmid

#21042

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus NB

Plasmid

#103986

Purpose

Plasmid encoding mVenus NB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus NB

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-Talin

Plasmid

#80026

Purpose

Mammalian expression of mCherry-Talin

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

Talin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGEP179_pX330K

Plasmid

#137882

Purpose

Entry vector for multi-gRNA cloning

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-N1

Plasmid

#79988

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-T7-OmpA-MNaseA-6xHis

Plasmid

#214808

Purpose

Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

Depositor

Peter Sarin

Article

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease A

Tags

6xhis

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET A-mEosEM-E

Plasmid

#226336

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; mEosEM-E for prokaryotic expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Insert

mEosEM-E

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-mGarnet2

Plasmid

#104312

Purpose

codes for a fusion of fluorescent protein mGarnet2 and human histon 2B (H2B), makes the H2B visible in live cells

Depositor

Ulrich Nienhaus

Article

Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

Insert

H2B-mGArnet2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDZ415 (24MS2SL loxP-Kan-loxP)

Plasmid

#45162

Depositor

Robert Singer, Daniel Zenklusen

Article

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

24xMS2 Stem Loop Cassette

Available Since

Aug. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pLVPT-tTR-KRAB

Plasmid

#11642

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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Cas9m4-VP64

Plasmid

#47319

Purpose

Cas9m4 Activator

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m4-VP64

Use

CRISPR

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pLVET-tTR-KRAB

Plasmid

#11644

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hEF-1alpha, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pJJB308

Plasmid

#107699

Purpose

AtU6 Promoter-LwaCas13a-gRNA Scaffold

Depositor

Daniel Voytas

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Type

Empty backbone

Expression

Plant

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-human Dynamin 1aa

Plasmid

#22163

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

D744N

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro dr_cPLA2-mKate2

Plasmid

#163844

Purpose

Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAGT9403

Plasmid

#192352

Purpose

L0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stop

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

TTCG_Stop-tNOS_CGCT

Expression

Plant

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pME-BrainbowTEC

Plasmid

#82405

Purpose

Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Inserts

tdTomatoMYC fusion
EGFP
E2CrimsonHA

Tags

HA and MYC

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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N-GFP-Dcp2

Plasmid

#163585

Purpose

express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

DCP2 promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-beta2-microglobulin

Plasmid

#15883

Depositor

Peter Mombaerts

Article

Ishii et al Curr Biol. 2003 Mar 4. 13(5):394-400.

Insert

beta2-microglobulin (B2m Mouse)

Available Since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only