We narrowed to 30,836 results for: Ide

1. pMK1221

   Plasmid

   #84220

   Purpose

   Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, mCherry

   Depositor

   Martin Kampmann, Jonathan Weissman

   Article

   Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. EcSTH

   Plasmid

   #211923

   Purpose

   This plasmid contains human codon optimized sequence of EcSTH which can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio in mammalian cells.

   Depositor

   Binghui Li

   Article

   Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

   Insert

   EcSTH
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS4333 yeTadA1.0-T7 RNAP

   Plasmid

   #137735

   Purpose

   pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

   Depositor

   Christina Smolke

   Article

   Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

   Insert

   yeTadA1.0-T7 RNAP
   

   Expression

   Bacterial and Yeast

   Mutation

   E366G (See depositor comment below)

   Available Since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6xHis-SNAP-PI Probe

   Plasmid

   #211507

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

   Depositor

   Hannes Maib, David Murray

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   BcPI-PLC-ANH
   

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST14-SpyCatcher

   Plasmid

   #35044

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyCatcher
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Deletion of C-terminal till I113, and point mutat…

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. EcSTH-LbNOX

   Plasmid

   #211924

   Purpose

   This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.

   Depositor

   Binghui Li

   Article

   Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

   Insert

   EcSTH-LbNOX
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. nls-transposase-nls-pA in pT3TS-Dest

   Plasmid

   #140873

   Purpose

   Vector for in vitro transcription of Tol2 nls-transposase-nls for zebrafish transgenesis using T3.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   nls-transposase-nls (Tol2)
   

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22-HT/plsA

   Plasmid

   #73625

   Purpose

   Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

   Depositor

   T. J. Kappock, James Williamson

   Article

   Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

   Insert

   adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-Jmjd3-F

   Plasmid

   #17440

   Purpose

   Expression of Jmjd3 in mammalian cells

   Depositor

   Kai Ge

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

   Insert

   JMJD3 (Kdm6b Mouse)
   

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-EGxxFP

    Plasmid

    #50716

    Purpose

    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    split EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRU5HT1-inLuc

    Plasmid

    #58954

    Purpose

    HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

    Depositor

    David Derse, Dmitriy Mazurov

    Article

    Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

    Insert

    inLuc
    

    Use

    Retroviral

    Promoter

    CMV/R/U5

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry-SopF

    Plasmid

    #135174

    Purpose

    Expresses an mCherry-SopF fusion in mammalian cells

    Depositor

    Leigh Knodler

    Article

    Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

    Insert

    SopF (STM1239 Salmonella enterica serovar Typhimurium)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJR152

    Plasmid

    #196280

    Purpose

    Cassette used in dual sgRNA library generation

    Depositor

    Rebecca Voorhees

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Insert

    hU6-sgRNA-CR3
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3-HA-human MYCN

    Plasmid

    #74163

    Purpose

    Mammalian expression of human MycN

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MycN (MYCN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEmpty_Control

    Plasmid

    #162607

    Purpose

    Empty control for Cas9 experiments and to clone new guide RNAs for paper

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Expression

    Yeast

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCas9n(D10A)-Blast

    Plasmid

    #63593

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, nickase mutant

    Promoter

    EFS-NS

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p6641 MSCV-P C-FlagHA 18E7-Kozak

    Plasmid

    #35019

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV18 E7 (E7 HPV 18)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    March 14, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pMo130-TelR

    Plasmid

    #50799

    Purpose

    Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

    Depositor

    Kim Lee Chua

    Article

    Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

    Insert

    tellurite-resistance cassette
    

    Use

    Suicide vector

    Promoter

    PCs12

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2-Tol1.zf1

    Plasmid

    #61388

    Purpose

    mRNA synthesis of tol1 transposase codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    tol1.zf1
    

    Use

    Zebrafish expression

    Promoter

    sp6

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGWB602-TP-mCherry-UnaG

    Plasmid

    #203483

    Purpose

    For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    Plastid transit peptide fused mCherry-UnaG
    

    Expression

    Bacterial and Plant

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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