We narrowed to 31,244 results for: LIS

1. pcDNA6-N-3XFLAG-Ctnnb1

   Plasmid

   #123586

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   catenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-mTurquoise2-IL-1beta-mNeonGreen

   Plasmid

   #166783

   Purpose

   FRET analysis (pro-IL-1beta cleavage) in living cells

   Depositor

   Hao Wu

   Article

   Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

   Insert

   mTurquoise2-IL-1b-mNeongreen (IL1B Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Spike-deltaC18 LB.1

   Plasmid

   #223277

   Purpose

   Encodes SARS-CoV-2 spike LB.1 for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike LB.1 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 SIRT7_H187Y

   Plasmid

   #53151

   Purpose

   expression vector for catalytically inactive human Sirtuin7

   Depositor

   Katrin Chua

   Article

   Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

   Insert

   Sirt7 (SIRT7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H187Y, catalytic inactive

   Promoter

   CMV

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. LacI-pLacI-pIV pm

   Plasmid

   #218101

   Purpose

   Phagemid that expresses LacI, and pIV under the Lac promoter

   Depositor

   Kevin Esvelt

   Insert

   pIV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-PPO-mScarlet

   Plasmid

   #139503

   Purpose

   Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Use

   Tags

   mScarlet following Gly-Gly-Ser linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

   Plasmid

   #224123

   Purpose

   Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-mutant (Q20A_Y23A_Y24F_F35A)
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Q20A_Y23A_Y24F_F35A in La(SSB)1-194

   Promoter

   CMV

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-puro-N-3HA-SREBP2-C-Flag

   Plasmid

   #134287

   Purpose

   Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP2 (Srebf2 Mouse)
   

   Use

   Lentiviral

   Tags

   3x HA and Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP2 precursor

   Promoter

   CMV

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA6-N-3XFLAG-Dvl2

   Plasmid

   #123587

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   dishevelled segment polarity protein 2 (Dvl2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIG-602_NY-ESO-1_TCR_RFP

    Plasmid

    #207483

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP, NY-ESO-1_TCR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197026

    Purpose

    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enOsCas12f1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D52R / T132R

    Promoter

    CBh, CMV, hU6

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNL-CEF-CD79B-RFP

    Plasmid

    #187005

    Purpose

    Expression of human CD79B as RFP fusion protein

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    CD79B (CD79B Human)
    

    Use

    Lentiviral

    Tags

    tagRFP

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

    Plasmid

    #65458

    Purpose

    Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TRIP12-V2_A755-A841

    Plasmid

    #194759

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    TRIP12 (TRIP12 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pRM053 His-TEV-Cas9 triNLS 0C

    Plasmid

    #196244

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-GFAP-TurboID

    Plasmid

    #227179

    Purpose

    Co-expression of FLAG-tagged-TurboID and T2A-mCherry under the GFAP short promoter by AAV vector

    Depositor

    Junmin Peng

    Article

    Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

    Insert

    TurboID
    

    Use

    AAV

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP_short

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA6-N-3XFLAG-Lrp6

    Plasmid

    #123595

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    low density lipoprotein receptor-related protein 6 (Lrp6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRM049 His-TEV-Cas9 triNLS 2C

    Plasmid

    #196245

    Purpose

    Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. single polypeptide FPX biosensor for caspase-3

    Plasmid

    #60883

    Purpose

    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-3
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIG-641 JE-TRAC-BCMA(BB)28z-P2A

    Plasmid

    #186127

    Purpose

    BCMA-CAR DNA production

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    BCMA-CAR (TNFRSF17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge