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pCMV-MbPylRS(DiZPK)

Plasmid

#91706

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

Depositor

P. Chen

Article

Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

Inserts

pylS
pylT

Expression

Mammalian

Mutation

T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Hygro empty (w214-1)

Plasmid

#17484

Purpose

3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223-ADPGK

Plasmid

#23809

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ADPGK (ADPGK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pBMPC3

Plasmid

#31367

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Bmal1 (Bmal1 Mouse)

Tags

His

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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Human ubiquitination-related proteins CRISPR KO library

Pooled Library

#174592

Purpose

Targets human ubiquitation-related proteins and deubiquitinating enzymes.

Depositor

Juan Bonifacino

Article

Jia et al Elife. 2019 Nov 6;8. pii: 50034. doi: 10

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only
pAAV_BiCHATe27_Gq_dTomato

Plasmid

#213832

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_Gq_dTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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UPRT-mCh-Nluc-P2A-neo-UPRT

Plasmid

#135015

Purpose

Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

mCh-Nluc-P2A-neo
UPRT 5'UTR
UPRT 3'UTR

Use

Cryptosporidium expression

Tags

mCherry and Nluc-neo

Promoter

Cryptosporidium actin and enolase

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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BL21ΔBC

Bacterial Strain

#102263

Purpose

lamB, ompC double knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
Murine E-cadherin mCherry

Plasmid

#71366

Purpose

mammalian expression of murine E-caderin with mCherry fusion

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

E-cadherin (Cdh1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FAS-Fc(DAPA)-AviTag-6xHis

Plasmid

#156541

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FAS (FAS Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9

Plasmid

#135964

Purpose

Expresses SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM228 - [Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR]

Plasmid

#159895

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR

Expression

Worm

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1

Plasmid

#12209

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-Pontin

Plasmid

#51635

Purpose

Mammalian expression of 3xFLAG tagged pontin, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

Pontin (RUVBL1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pIRESpuroN3xFLAG-UFSP2 C302A

Plasmid

#204554

Purpose

Expresses 3xFLAG-tagged UFSP2 C302A in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFSP2 (UFSP2 Human)

Expression

Mammalian

Mutation

C302A

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET Trx His6 LIC cloning vector (2Tc-T)

Plasmid

#37239

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis and Thioredoxin (Trx)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pKanB

Plasmid

#223523

Purpose

Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Depositor

Geoff Lin-Cereghino

Article

Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

Type

Empty backbone

Expression

Yeast

Promoter

AOX1

Available Since

Sept. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-BID(D59E)

Plasmid

#21133

Depositor

Junying Yuan

Article

Li et al Cell. 1998 Aug 21. 94(4):491-501.

Insert

Flag-tagged Mutant mouse BID

Tags

Flage

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
Bidirectional MYC 5'UTR Dual Fluorescence Reporter

Plasmid

#229498

Purpose

This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

MYC 5'Untranslated region + destabilized GFP

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCMU-ACTFr

Plasmid

#61191

Purpose

Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtFimbrin1-ABD

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

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