We narrowed to 5,551 results for: crispr cas9 grna plasmid

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pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-2

Plasmid

#237891

Purpose

Cre-KO AAV vector#2 containing SaCas9 and its sgRNA targeting Cre

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

sgRNA-Cre

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-3

Plasmid

#237892

Purpose

Cre-KO AAV vector#3 containing SaCas9 and its sgRNA targeting Cre

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

sgRNA-Cre

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dFnCas9

Plasmid

#201954

Purpose

Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dFnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A and H969A on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

Plasmid

#210709

Purpose

This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN

Use

AAV and CRISPR

Expression

Mammalian

Mutation

N55K in MCP

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pFREE

Plasmid

#92050

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Expression

Bacterial

Promoter

pRhamBAD and ptet

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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px459ver2.0-FKBP8-gRNA1 (for mouse)

Plasmid

#253968

Purpose

CRISPR/Cas9 (PX459) plasmid expressing gRNA N-terminal knock-in of mouse Fkbp8.

Depositor

Yusuke Hirabayashi

Article

Nakamura et al Nat Commun. 2025 Apr 17;16(1):3401. doi:

Insert

Fkbp8 (Fkbp8 Mouse)

Use

CRISPR

Promoter

U6

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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PX459v2-ILK-gRNA 1-exon 1

Plasmid

#163320

Purpose

SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ILK gRNA 1_Exon1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
PX459v2-ILK-gRNA 2-exon 8

Plasmid

#163321

Purpose

SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ILK gRNA 2_Exon8

Use

CRISPR

Expression

Mammalian

Promoter

U6F

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

Plasmid

#163030

Purpose

Expression of CRISPRi with gSTOP gRNA

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 pA, and myc NLS

Expression

Mammalian

Promoter

Alb-AAT

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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LMX1B-gRNA-N-del

Plasmid

#131333

Purpose

gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

LMX1B-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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