166,542 results

1. pCAG-tTA

   Plasmid

   #104102

   Purpose

   Expresses tTA2 under the CAG promoter in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

   Insert

   tTA2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUBC-SV40NLS-Pfv-Sapphire

   Plasmid

   #203653

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV8)

   Viral Prep

   #105530-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CMV

   Tags

   EGFP

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

4. aeBlue chromoprotein

   Plasmid

   #117846

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, aeBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Twinkle-APEX2-V5

   Plasmid

   #129705

   Purpose

   APEX2 fused to the mitochondrial nucleoid protein Twinkle

   Depositor

   Alice Ting

   Article

   Han et al Cell Chem Biol. 2017 Mar 16;24(3):404-41

   Insert

   Twinkle-APEX2-V5 (TWNK, APEX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1 hSCN5A

   Plasmid

   #145374

   Purpose

   expresses human Nav1.5 (WT) in mammalian cells

   Depositor

   Robert Kass, Hugues Abriel

   Article

   Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

   Insert

   sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mPiezo1-IRES-eGFP

   Plasmid

   #80925

   Purpose

   expresses mouse Piezo1 with GFP

   Depositor

   Ardem Patapoutian

   Article

   Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

   Insert

   mouse Piezo1 CDS (Piezo1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. His6-TEV-CRBN-midi

   Plasmid

   #215330

   Purpose

   CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

   Depositor

   Alessio Ciulli

   Article

   Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

   Insert

   CRBN (CRBN Human)
   

   Use

   Tags

   6xHis and TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 41-187 followed by a Gly-Ser…

   Promoter

   lacI

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVP91A-pcaHG

   Plasmid

   #113766

   Purpose

   Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

   Depositor

   John Lipscomb

   Article

   Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

   Inserts

   β subunit of Protocatechuate 3,4- Dioxygenase
   α subunit of Protocatechuate 3,4- Dioxygenase
   

   Use

   Tags

   Met-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. mClY-N1

    Plasmid

    #90457

    Purpose

    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

    Insert

    mClY-N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

    Promoter

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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