167,785 results

1. Calnexin-HT8-mGold

   Plasmid

   #183989

   Purpose

   Expresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.

   Depositor

   Pablo Rivera-Fuentes

   Article

   Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

   Insert

   Calnexin (CANX Human)
   

   Tags

   HaloTag8 (C61S, C262S), mGold

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-HA-Nrxn1beta AS4(+)

   Plasmid

   #59410

   Purpose

   Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Nrxn1beta AS4(+) (Nrxn1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_CMV: Flag-Antares_SV40:PuroR

   Plasmid

   #183049

   Purpose

   Expresses FLAG-tagged Antares in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

   Insert

   Antares BRET reporter
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRG205MX

   Plasmid

   #64535

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-Con/Foff 2.0-mCherry

   Plasmid

   #137133

   Purpose

   Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRG206MX

   Plasmid

   #64536

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-hCDC6p-Venus-puro

   Plasmid

   #212666

   Purpose

   Express Venus under CDC6 promoter

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   hCDC6p-Venus
   

   Use

   Lentiviral

   Promoter

   hCDC6p

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 HA E2F1

   Plasmid

   #236431

   Purpose

   transient overexpression of E2F1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   E2F1 (E2F1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-Ef1a-hMGT-PGK-puro

   Plasmid

   #172394

   Purpose

   Lentiviral expression vector for human direct cardiac reprogramming

   Depositor

   Li Qian

   Article

   Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

   Insert

   Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
   

   Use

   Lentiviral

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIC162

    Plasmid

    #63070

    Purpose

    Retroviral expression of EGFP-TEV-S-tag Nuf2 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    Nuf2 (NUF2 Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pYHY2

    Plasmid

    #216108

    Purpose

    Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.

    Depositor

    Shannon Sirk

    Article

    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

    Insert

    NanoLuc
    

    Use

    Synthetic Biology; E. coli-bacteroides shuttle ve…

    Tags

    3xFLAG tag and BT0294 signal peptide

    Expression

    Bacterial

    Promoter

    BfP1E6 promoter

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PT3.5/CMV-EGFRvIII

    Plasmid

    #20280

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    EGFRvII (EGFR Human)
    

    Use

    Transposon

    Mutation

    See depositor comments below.

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. mVenus-pBAD

    Plasmid

    #54845

    Purpose

    Localization: Protein Expression Vector , Excitation: 515, Emission: 528

    Depositor

    Atsushi Miyawaki, Michael Davidson

    Article

    Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

    Type

    Empty backbone

    Tags

    6xHis and mVenus

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Opie2-PuroR

    Plasmid

    #131613

    Purpose

    Expresses PuroR and dsRed.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR
    dsRed
    

    Expression

    Insect

    Promoter

    Opie2 and hr5Ie1

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJJS002

    Plasmid

    #188325

    Purpose

    PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::GFP::mIAA7
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS1NB m Rosella I

    Plasmid

    #71247

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Tags

    yeast mitochondrial targeting sequence of citrate…

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV4 p50

    Plasmid

    #21965

    Depositor

    Warner Greene

    Article

    Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

    Insert

    NF-kB p50 subunit (NFKB1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGT-Ah7

    Plasmid

    #122574

    Purpose

    MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    catP/sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGE-attBTT-loxP-DsRed (pTL780)

    Plasmid

    #115160

    Purpose

    Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    attB-TT loxP 3xP3-DsRed
    

    Use

    Cre/Lox

    Expression

    Insect

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

    Plasmid

    #120563

    Purpose

    Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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