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pBA1326

Plasmid

#246058

Purpose

Bacterial expression of RFP under control of the pJEX promoter

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Red Fluorescent Protein

Expression

Bacterial

Promoter

pJEX promoter

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD_SynPspA

Plasmid

#223814

Purpose

Bacterial expression of SynPspA with C-terminal YFP

Depositor

Carsten Sachse

Article

Junglas et al Cell. 2021 Jul 8;184(14):3674-3688.e18.

Insert

SynPspA

Tags

YFP

Expression

Bacterial

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET50b_SynPspA

Plasmid

#223813

Purpose

Bacterial expression of SynPspA with N-terminal NusA-tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Cell. 2021 Jul 8;184(14):3674-3688.e18.

Insert

SynPspA

Tags

NusA

Expression

Bacterial

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pNA-Y

Plasmid

#238993

Purpose

Node A carrying sgRNA-1 that can be used for the assembly of pEND-Y11

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

sgRNA-1

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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1595-pMV-arsR-ABS

Plasmid

#240494

Purpose

Arsenic Biosensor

Depositor

Jan Roelof van der Meer

Article

Wackwitz et al Microb Biotechnol. 2008 Mar;1(2):149-57.

Insert

ABS-arsR-ABS

Expression

Bacterial

Mutation

Second ABS after arsR gene

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET29a-α3-E

Plasmid

#187935

Purpose

Express phage alpha3 lysis protein E in bacteria

Depositor

Yi Ma

Article

Ma et al Bioengineering (Basel). 2022 Sep 2;9(9):

Insert

Phage alpha 3 lysis protein E

Tags

Strep II

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-Z11

Plasmid

#239001

Purpose

Final circuit with -Ara: ON, +Ara: ON behaviour

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

Circuit with sfGFP expression (-Ara: ON, +Ara: ON)

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSIG2

Plasmid

#226909

Purpose

MoClo-compatible vectors for dsRNA production in E. coli

Depositor

Li Hung Chen

Article

Wu et al Plant Methods. 2025 Jul 11;21(1):96. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_003

Plasmid

#210760

Purpose

SC_Hygromycin phosphotransferase

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

Hygromycin phosphotransferase

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry_I3

Plasmid

#239340

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with mCherry in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mCherry

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pmBaoJin_I3

Plasmid

#239337

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with mBaoJin in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mBaoJin

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pmRuby3_I3

Plasmid

#239344

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with mRuby3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mRuby3

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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DriD-wHTH-DNABD

Plasmid

#237851

Purpose

The wHTH DNA binding domain used to understand the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD wHTH DNABD

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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DriD-3HB-mutant

Plasmid

#237852

Purpose

DriD 3HB surface mutant used to interrogate the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD 3HB mutant

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHK-cinI

Plasmid

#235486

Purpose

Inducible gene cinI for OC14-HSL production by Ptac promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

cinI

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.6

Plasmid

#235471

Purpose

Message phagemid carrying sgRNA6 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA6

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK052.3

Plasmid

#235472

Purpose

Message phagemid carrying sgRNA3 (prom. J23119, backbone RSF1030)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK002.1

Plasmid

#235460

Purpose

Message phagemid carrying sgRNA1 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA1

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK002.3

Plasmid

#235462

Purpose

Message phagemid carrying sgRNA3 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK002.4

Plasmid

#235463

Purpose

Message phagemid carrying sgRNA4 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA4

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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