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pAG416GAL-EMCV_3C

Plasmid

#201941

Purpose

Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

EMCV 3C protease (EMCVgp1 )

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-MARK3_T211A

Plasmid

#107236

Purpose

Lentiviral gene expression vector for phospho-MARK3_T221A expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-HRV-B14_3C

Plasmid

#203466

Purpose

Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HRV-B14 3C protease (HRVBgp1 )

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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SBC009876

Plasmid

#226283

Purpose

Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

Depositor

Nigel Scrutton

Article

Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1

Expression

Bacterial

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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CLK2

Plasmid

#155459

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLK2 (CLK2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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H14-MBP-SUMO-Cys-Linker-BAF_G47E

Plasmid

#101779

Purpose

Expresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Tags

His14-MBP-SUMO

Expression

Bacterial

Mutation

G47E mutation

Promoter

T5

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE2 mCherry-Rtn4HD

Plasmid

#86685

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4 homology domain (RTN4 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

homology domain only

Promoter

CMV

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-Halo-hMRE11(H129N)

Plasmid

#208394

Purpose

To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human MRE11 (MRE11 Human)

Use

Luciferase and Retroviral

Expression

Mammalian

Mutation

H129N

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1

Plasmid

#218524

Purpose

sgRNA targeting human KIAA0141/DELE1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

DELE1 (DELE1 Human)

Use

CRISPR and Lentiviral

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2

Plasmid

#218525

Purpose

sgRNA targeting human KIAA0141/DELE1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

DELE1 (DELE1 Human)

Use

CRISPR and Lentiviral

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.DMAP1

Plasmid

#184650

Purpose

Retroviral expression of mouse DMAP1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Dmap1 (Dmap1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2 GFP-Rtn4HD

Plasmid

#86686

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4 homology domain (RTN4 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

homology domain only

Promoter

CMV

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-1574/+120

Plasmid

#35539

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Promoter

none

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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DDX11

Plasmid

#155502

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX11 (DDX11 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (1-90) (72P)

Plasmid

#62080

Purpose

Expression of human p53 (1-90) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

contains TAD/PP residues 1-90; 72P

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pEN_TT 3xFLAG-MAVS

Plasmid

#158628

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Nkx2.5

Plasmid

#72689

Purpose

Doxycyline regulated expression of Nkx2.5 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Nkx 2.5 (Nkx2-5 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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shTCF7L2 # 2

Plasmid

#42558

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTCF7L2 (TCF7L2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
DCP2

Plasmid

#155499

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DCP2 (DCP2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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