We narrowed to 36,418 results for: Tec

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pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

Plasmid

#200126

Purpose

Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG.Myc-SARS2_3CL(C145A)

Plasmid

#200121

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Tags

Myc

Expression

Mammalian

Mutation

C145A

Promoter

CAG

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196081

Purpose

Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196083

Purpose

Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196075

Purpose

Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196077

Purpose

Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Hs4EBP3_36-69-L61A V67A-GB1_AB

Plasmid

#148380

Purpose

Bacterial Expression of Hs4EBP3_36-69-L61AV67A

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP3_36-69-L61AV67A (EIF4EBP3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Hs4EBP3_36-69-Y40AL45A-GB1_AA

Plasmid

#148280

Purpose

Bacterial Expression of Hs4EBP3_36-69-Y40AL45A

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP3_36-69-Y40AL45A (EIF4EBP3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Hs4EBP3_36-69-YLLVtoAAA-GB1_AA

Plasmid

#148281

Purpose

Bacterial Expression of Hs4EBP3_36-69-YLLVtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP3_36-69-YLLVtoAAAA (EIF4EBP3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-F41DW42D_AB

Plasmid

#148372

Purpose

Insect Expression of Dm4E-T-F41DW42D

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T-F41DW42D (4E-T Fly)

Expression

Insect

Mutation

5 non silent mutation L127S, E493G, H628R, N659S,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-YLFWtoAADD_AB

Plasmid

#148373

Purpose

Insect Expression of Dm4E-T-YLFWtoAADD

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T-YLFWtoAADD (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations H611R and N659S and 8 si…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_32-83-GB1_AA

Plasmid

#148267

Purpose

Bacterial Expression of DmThor_32-83

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_32-83 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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CABE-T1.14

Plasmid

#193259

Purpose

Expression of CABE-T1.14 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T1.14

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.31

Plasmid

#193281

Purpose

Expression of CBE-T1.31 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.31

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.32

Plasmid

#193282

Purpose

Expression of CBE-T1.32 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.32

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.45

Plasmid

#193283

Purpose

Expression of CBE-T1.45 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.45

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.53

Plasmid

#193286

Purpose

Expression of CBE-T1.53 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.53

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CABE-T1.19

Plasmid

#193261

Purpose

Expression of CABE-T1.19 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T1.19

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CABE-T2.5

Plasmid

#193262

Purpose

Expression of CABE-T2.5 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T2.5

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CABE-T2.23

Plasmid

#193266

Purpose

Expression of CABE-T2.23 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T2.23

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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