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pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

Plasmid

#205269

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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NbV5-mCherry

Plasmid

#201474

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-NRF1-HA

Plasmid

#34708

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag and HA

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Epac2

Plasmid

#118314

Purpose

Expression of mouse Epac2

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2008 Dec;28(23):7109-25.

Insert

Epac2 (Rapgef4 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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NbV5-GFP2

Plasmid

#201477

Purpose

BRET2 detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-GFP2

Tags

GFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

Plasmid

#233445

Purpose

PiggyBac donor plasmid to overexpress a full length ADAR2

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBF3038

Plasmid

#26850

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Insert

CreA recombinase

Use

Cre/Lox

Expression

Yeast

Mutation

N/A

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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NbV5-HALOTAG

Plasmid

#201482

Purpose

Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-HALOTAG

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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NbV5-HiBiT

Plasmid

#201489

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-HiBiT

Tags

HiBit

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV4-NIK-HA

Plasmid

#27554

Depositor

Shao-Cong Sun

Article

Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

Insert

NIK (MAP3K14 Human)

Tags

HA

Expression

Mammalian

Mutation

NIK starts at amino acid 3; C52R and R139H mutati…

Promoter

CMV

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
Rluc8-NbV5

Plasmid

#201479

Purpose

BRET detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-Rluc8

Tags

Rluc8

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PcDNA3.1-2Strep

Plasmid

#210343

Purpose

Empty Vector for molecular cloning

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

2Strep tag

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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GFP2-NbALFA

Plasmid

#201486

Purpose

BRET2 detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

GFP2-NbALFA

Tags

GFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-NbV5

Plasmid

#201490

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

HiBiT-NbV5

Tags

HiBit

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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p3x-FLAG-hnRNPF

Plasmid

#21926

Depositor

Mariano Garcia-Blanco

Article

Mauger et al Mol Cell Biol. 2008 Sep . 28(17):5403-19

Insert

hnRNPF (HNRNPF Human)

Tags

3 Flags

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only

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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Article

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pSEM246 - MCS mosTI unc-119 target

Plasmid

#159821

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI unc-119 target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

Plasmid

#205268

Purpose

Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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PX552-mScn2a-3xV5-EF1-smFP-flag

Plasmid

#182561

Purpose

For mouse Nav1.2 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn2a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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