We narrowed to 22,759 results for: ESC;

1. pSF279

   Plasmid

   #167674

   Purpose

   Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TurboRFP-pBAD

   Plasmid

   #54858

   Purpose

   Localization: Protein Expression Vector , Excitation: 553, Emission: 574

   Depositor

   Dmitriy Chudakov, Michael Davidson, Dorus Gadella

   Article

   Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

   Type

   Empty backbone

   Tags

   6xHis and TurboRFP

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. Sirius-mT-Sapphire_pcDNA3

   Plasmid

   #89584

   Purpose

   Genetically encoded ratiometric fluorescent thermometer

   Depositor

   Takeharu Nagai

   Article

   Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

   Insert

   Sirius-mT-Sapphire
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nano-lantern(cAMP-1.6)/pcDNA3

   Plasmid

   #53594

   Purpose

   Nano-lantern-based luminescent cAMP indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern-based luminescent cAMP indicator
   

   Expression

   Mammalian

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Nano-lantern(cAMP-1.6)/pRSETB

   Plasmid

   #53591

   Purpose

   Nano-lantern-based luminescent cAMP indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern-based luminescent cAMP indicator
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pMuLE_ENTR_CBF-tdTomato_L3-L2

   Plasmid

   #113708

   Purpose

   Single pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Insert

   CBF (notch dependent promoter)
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRU325

   Plasmid

   #167676

   Purpose

   Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pluc2-iRFP720

   Plasmid

   #98887

   Purpose

   Mammalian expression

   Depositor

   Laura Mezzanotte

   Article

   Mezzanotte et al Cell Transplant. 2017 Apr 26. doi: 10.37

   Insert

   Firefly Luciferase
   

   Tags

   iRFP720

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_LifeAct-iBlueberry

   Plasmid

   #73176

   Purpose

   Expressing iBlueberry and mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

   Insert

   LifeAct-iBlueberry
   

   Expression

   Mammalian

   Mutation

   mutation E177Q in iBlueberry

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mApple-hCOL1A1

    Plasmid

    #140113

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

    Plasmid

    #182009

    Purpose

    CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    IgKchL_HA_STf_myc_PDGFRtmb
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mVenus-hCOL1A1

    Plasmid

    #140111

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

    Plasmid

    #140168

    Purpose

    Expresses a regulatable secretory cargo for Tetrahymena thermophila

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Other

    Promoter

    MTT1

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_NES_STf

    Plasmid

    #182001

    Purpose

    CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    NES-STf
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-smURFP-T2A-mCherry

    Plasmid

    #80347

    Purpose

    Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCherry.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    smURFP
    mCherry
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMABC

    Plasmid

    #45398

    Depositor

    Katsu Murakami

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Inserts

    rpoA (rpoA Escherichia coli)
    rpoB (rpoB Escherichia coli)
    rpoC (rpoC Escherichia coli)
    

    Expression

    Bacterial

    Promoter

    None and T7

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCerulean-hCOL1A1

    Plasmid

    #140112

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXint129

    Plasmid

    #178208

    Purpose

    Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

    Depositor

    Gregory Phillips

    Article

    Platt et al Plasmid. 2000 Jan . 43(1):12-23.

    Insert

    xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
    

    Expression

    Bacterial

    Promoter

    lacI

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. mMaroon-PAK-mMaroon

    Plasmid

    #84361

    Purpose

    FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-PAK-mMaroon
    

    Tags

    mMaroon flanking PAK domain on N and C terminus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. mMaroon-RTK-mMaroon

    Plasmid

    #84359

    Purpose

    FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-Rtk--mMaroon
    

    Tags

    mMaroon flanking Rtk domain on N and C terminus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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