Skip to main content
Addgene
Showing: 821 - 840 of 5573 results

  1. OpenLoopControl

    Plasmid
    #59895
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTR
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST
    Expression
    Mammalian
    Mutation
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59895-plasmid-map-sequence-id-108253

    Enlarge

  16. pOpen-BsupolLF

    Plasmid
    #165563
    Purpose
    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    Bsu DNA Polymerase I, Large Fragment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165563-plasmid-map-sequence-id-340036

    Enlarge

  20. pBN095

    Plasmid
    #173765
    Purpose
    express E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    delete motif 3
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    173765-plasmid-map-sequence-id-344806

    Enlarge

Showing: 821 - 840 of 5573 results