We narrowed to 18,683 results for: met

1. Twitch-4 pcDNA3

   Plasmid

   #49533

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-4
   

   Use

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

2. Twitch-1 pcDNA3

   Plasmid

   #49812

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-1
   

   Use

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCAG_EGFP-RandE3

   Plasmid

   #79870

   Purpose

   Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Beta-actin

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-BFP

   Plasmid

   #106282

   Purpose

   A version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.

   Depositor

   Jay Shendure

   Article

   Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

   Insert

   CMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcPylRS(Y384F Y306A)

   Plasmid

   #196486

   Purpose

   To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.

   Depositor

   Kensaku Sakamoto

   Article

   Hino et al Methods Mol Biol. 2012;794:215-28. doi:

   Insert

   pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p168

   Plasmid

   #62618

   Purpose

   ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR

   Depositor

   Mattheos Koffas

   Article

   Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

   Inserts

   flavaone 3B-hydroxylase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   

   Use

   Synthetic Biology

   Tags

   GBD, PDZ, and SH3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Twitch-2C pcDNA3

   Plasmid

   #104501

   Purpose

   fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-2C
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAFMW-AthHEN1

   Plasmid

   #104980

   Purpose

   Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Insert

   Hen1 (HEN1 Mustard Weed)
   

   Use

   Tags

   FLAG Myc

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p148

   Plasmid

   #62616

   Purpose

   ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR

   Depositor

   Mattheos Koffas

   Article

   Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

   Inserts

   flavaone 3B-hydroxylase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   

   Use

   Synthetic Biology

   Tags

   GBD, PDZ, and SH3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hSTARR-seq_SCP1 vector_blocking 3

    Plasmid

    #99318

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p158

    Plasmid

    #62617

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hSTARR-seq_OCT4 3UTR vector

    Plasmid

    #99295

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hSTARR-seq_mCP vector

    Plasmid

    #99291

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hSTARR-seq_SCP1 vector_blocking 1

    Plasmid

    #99316

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hSTARR-seq_SULT1C vector

    Plasmid

    #99294

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hSTARR-seq_TTF2 vector

    Plasmid

    #99293

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAFMW-AthHEN1-CM

    Plasmid

    #104981

    Purpose

    Expression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Insert

    Hen1 (HEN1 Mustard Weed)
    

    Use

    Tags

    FLAG Myc

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSTARR-seq_SCP1 vector_blocking 2

    Plasmid

    #99317

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ucp1-empty-miR122

    Plasmid

    #231695

    Purpose

    Empty control vector for pAAV-Ucp1-Cre-miR122

    Depositor

    Joerg Heeren

    Article

    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Ucp1 enhancer-promoter

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. CROP-seq-opti

    Plasmid

    #106280

    Purpose

    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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