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pHD1

Plasmid

#30974

Depositor

Adam Bogdanove, Daniel Voytas

Publication

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat HD1

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mito).cpSFGFP.HaloTag

Plasmid

#214925

Purpose

Expresses mitochondrially targeted non-responsive control

Depositor

Timothy Ryan

Publication

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).cpSFGFP.HaloTag

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBiFCt-2in1-CC

Plasmid

#105114

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Publication

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Tags

cYFP-Myc/nYFP-HA

Expression

Plant

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTRE-EGFP

Plasmid

#89871

Purpose

EGFP reporter vector for iTango system

Depositor

Hyungbae Kwon

Publication

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

tetO-EGFP

Expression

Mammalian

Promoter

TetO

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1-IDH2-R172K

Plasmid

#87927

Purpose

Expresses IDH2 (R172K mutant) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Publication

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Tags

none

Expression

Mammalian

Mutation

R172K

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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PA-DAD-wt

Plasmid

#53095

Purpose

Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.

Depositor

Klaus Hahn, Ronen Zaidel-Bar

Publication

Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

Insert

LOV(wt)-6aa-DAD

Tags

6x His and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

L47F and C49S in Venus

Available since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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anti-Notch1_E6-pBIOCAM5

Plasmid

#39344

Depositor

John McCafferty

Publication

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch1 (clone B9) (Notch1, NOTCH1 Mouse, Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLKO.1 - TRC mTagBFP2

Plasmid

#191566

Purpose

Expresses an shRNA and mTagBFP2

Depositor

Timothy Ryan

Publication

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMaCTag-P34

Plasmid

#120030

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCS-membrane-ceruleanFP

Plasmid

#53749

Purpose

Contains membrane localized cerulean fluorescent protein in pCS expression vector.

Depositor

Sean Megason

Publication

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

2x membrane linker

Tags

Cerulean

Expression

Mammalian

Promoter

simian CMV IE94

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTH-PanCen-mVenus

Plasmid

#49640

Purpose

TALEColor-mVenus-Pan-Centromere

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Type

Empty backbone

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG:myr-Venus

Plasmid

#32602

Depositor

Anna-Katerina Hadjantonakis

Publication

Rhee et al Genesis. 2006 Apr;44(4):202-18.

Insert

myr-Venus

Expression

Mammalian

Promoter

CAG

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
ptubP-QS

Plasmid

#24352

Depositor

Liqun Luo

Publication

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QS

Tags

white gene

Expression

Insect

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Myr.PKCzeta.FLAG

Plasmid

#10802

Depositor

Alex Toker

Publication

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG and Myr

Expression

Mammalian

Available since

July 26, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pYOY152

Plasmid

#13987

Depositor

Linda Wordeman

Publication

Ovechkina et al J Cell Biol. 2002 Nov 25. 159(4):557-62.

Insert

Mitotic Centromere Associated Kinesin

Tags

GFP and His

Expression

Mammalian

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Puro_siKO

Plasmid

#86696

Purpose

Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Publication

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 TO

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn1-tTA

Plasmid

#99119

Purpose

An AAV genome that expresses tTA from the hSyn1 promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

hSyn1

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJWV102-PL-dCas9

Plasmid

#85588

Purpose

Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

Depositor

Jan-Willem Veening

Publication

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

dCas9sp

Use

CRISPR

Expression

Bacterial

Promoter

PLsp

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBS mCD4

Plasmid

#14613

Depositor

Dan Littman

Publication

Landau et al Nature. 1988 Jul 14. 334(6178):159-62.

Insert

CD4 (Cd4 Mouse)

Expression

Bacterial

Available since

June 27, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209653

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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