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pBOB-N-Flag-GK (12-272)

Plasmid

#134272

Purpose

Lentivector encoding Flag-tagged GK truncation (amino acids 12-272)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk amino acids 12-272

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5-toku (1-174)

Plasmid

#134261

Purpose

Lentivector encoding Flag-tagged toku form of GK5 (amino acids 1-174)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 1-174

Promoter

CMV

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D30N+D280N)

Plasmid

#134292

Purpose

Expresses GK5 (isoform 2)-D30N/D280N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acids 30 and 280 to Asparagine

Promoter

T7

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-C-Flag-GK5_v1

Plasmid

#134281

Purpose

Expresses Flag-tagged GK5 (isoform 1) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (416-534)

Plasmid

#134267

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 416-534)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 396-534

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (396-534)

Plasmid

#134266

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 396-534)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 396-534

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (287-534)

Plasmid

#134264

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 287-534)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 287-534

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (25-287)

Plasmid

#134263

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 25-287)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 25-287

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (1-287)

Plasmid

#134262

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 1-287)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 1-287

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (287-396)

Plasmid

#134265

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 287-396)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 287-396

Promoter

CMV

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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SQ171/pCSacB/ptRNA100

Bacterial Strain

#155206

Purpose

SQ171 strain supported by pCSacB and ptRNA100 plasmids

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Bacterial Resistance

Kanamycin

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only
BARBEKO sgRNA library

Pooled Library

#174163

Purpose

Cytosine base editors for genome-scale knockout screens in human cells. Targets gene start codons, splice sites, or introduces premature termination codons.

Depositor

Wensheng Wei

Article

Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only
pUC57kan-T7-mt-gRNA library

Pooled Library

#82480

Purpose

This library is intended to be used as a technical tool for removal of mitochondrial DNA from mouse genomic DNA preparations.

Depositor

Wei Xie

Article

Wu et al Nature. 2016 Jun 30;534(7609):652-7.

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only
SB100X in pCAG globin pA

Plasmid

#127909

Purpose

Sleeping Beauty Transposase expression vector driven by the CAG promoter

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

Sleeping Beauty Transposase

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SEP-Glur2 (MCA10)

Plasmid

#64941

Purpose

mammalian expression of rat GluR2

Depositor

Jeremy Henley

Article

Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

Insert

GluR2 (Gria2 Rat)

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFZ19

Plasmid

#36184

Purpose

Expression of genes under control of an estrogen-inducible 35S promoter

Depositor

Daniel Voytas

Article

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Expression

Plant

Promoter

XVE_35S (estrogen-inducible)

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#138149

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NLS-HA

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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lenti F9-TetR-EGFP-IRES-PuroR

Plasmid

#117049

Purpose

lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Insert

F9-TetR-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

F9 promoter

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pUHD.NFATc1nuc

Plasmid

#40616

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1nuc (NFATC1 Human)

Tags

HA

Expression

Mammalian

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Promoter

CMV2

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST12.2 DYRK2 WT

Plasmid

#20005

Depositor

Anjana Rao

Article

Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

Insert

DYRK2 (DYRK2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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