We narrowed to 30,916 results for: TRO

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F40 tension sensor

Plasmid

#101252

Purpose

Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

Depositor

Carsten Grashoff

Article

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-F40-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-NLS-cTF

Plasmid

#87746

Purpose

IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and NLS linker - cTF (chimeric transcriptio…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV PIG Erg

Plasmid

#66984

Purpose

Retroviral expression plasmid for mouse Erg expression; containing bicistronic IRES-GFP

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (Erg Mouse)

Use

Retroviral

Promoter

LTR

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mDIS3-FLAG

Plasmid

#60046

Purpose

Mammalian expression of FLAG-tagged mouse DIS3

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

DIS3 (Dis3 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
pCFJ1202

Plasmid

#44484

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, MCS

Use

Multiple cloning site vector

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCX4-Oct4

Plasmid

#36116

Depositor

Steven Dowdy

Article

Israel et al Nature. 2012 Jan 25;482(7384):216-20. do

Insert

Oct4 (POU5F1 Human)

Use

Retroviral

Promoter

CMV

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-KSR2

Plasmid

#23501

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

KSR2 (KSR2 Human)

Use

Gateway Cloning

Mutation

aa344-5 (DP) deleted; S523G compared to GenBank E…

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-TTK

Plasmid

#23857

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TTK (TTK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWPI-N1ICD

Plasmid

#185525

Purpose

Constitutive overexpression of human NOTCH1 intracellular domain

Depositor

Sean Palecek, Eric Shusta

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

NOTCH1 intracellular domain (NOTCH1 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Venus/Cerulean-Synaptopodin-A in pcDNA6

Plasmid

#190798

Purpose

Imaging of synaptopodin in live cells

Depositor

Vivian Tang

Article

Morris et al J Cell Biol. 2022 May 2;221(5). pii: 213

Insert

Synaptopodin-A (SYNPO Synthetic, Human)

Tags

Cerulean, His, Venus, and myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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RKS-Fzd7 subtype NGS Wnt

Plasmid

#159626

Purpose

Express Fzd7 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1 TMEM192-Flag-FKBP

Plasmid

#112743

Purpose

express TMEM192-Flag-FKBP

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

TMEM192-Flag-FKBP (TMEM192 Human)

Use

Lentiviral

Tags

Flag, FKBP

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-LZ-CD95L

Plasmid

#104349

Purpose

Expresses human CD95L leucine zipper in mammalian cells

Depositor

Alexander McLellan

Article

Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

Insert

CD95L leucine zipper (FASLG Human)

Tags

octahistidine tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-WIF1

Plasmid

#99533

Purpose

WIF1 expression plasmid

Depositor

Qian Tao

Article

Chan et al Lab Invest. 2007 Jul;87(7):644-50. Epub

Insert

WIF1 (WIF1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMRXIP Lamp1-Venus

Plasmid

#89937

Purpose

Expresses Lamp1 tagged with Venus in mammalian cells

Depositor

Noboru Mizushima

Article

Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

Insert

rat Lamp1 (Lamp1 Rat)

Use

Retroviral

Tags

Venus

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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h-Plekhm1_FLAG-pcDNA 3.1

Plasmid

#73593

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK30

Plasmid

#59121

Purpose

FAK fret sensor with 30 nm helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX3x-hRanBP2-(IR1+M)

Plasmid

#53140

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

RanBP2 fragment (RANBP2 Human)

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2631-2711)

Promoter

tac

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-Nras61K-Puro

Plasmid

#49404

Purpose

MSCVpuro based vector, expressing Nras mutant 61K.

Depositor

Tobias Neff

Insert

N Ras K61 (NRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

61K constitutively activated

Promoter

MSCV promotor

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt11-V5

Plasmid

#35941

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt11 (wnt11f2 Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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