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pLVX-UBE3BΔIQ-HA-IRES-Puro

Plasmid

#107350

Purpose

Expresses HA tagged UBE3B deletion of IQ domain in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3BdelIQ (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78306

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(chimpanzee)

Plasmid

#79032

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(chimpanzee)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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2-Luc-Del-50

Plasmid

#21730

Depositor

Wafik El-Deiry

Article

Zeng et al Nat Genet. 1997 Jan . 15(1):78-82.

Insert

p21 promoter deletion mutant (CDKN1A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Identical to 2-Luc except that the 50-bp region b…

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pGP-CMV-NES-fRGECO1

Plasmid

#125247

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Tags

NES signal

Expression

Mammalian

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-HNHx-ABEmax7.10HD-BlastR

Plasmid

#176477

Purpose

A-to-G base editor with alternative targeting scope

Depositor

Gerald Schwank

Article

Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

Insert

HNHx-ABEmax7.10HD

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-LMP2A

Plasmid

#195816

Purpose

Gateway Entry Clone of Epstein-Barr virus LMP2A

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

LMP2A (LMP-2A Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BNLF2a

Plasmid

#195767

Purpose

Gateway Entry Clone of Epstein-Barr virus BNLF2a

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BNLF2a (BNLF2a Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BNLF2b

Plasmid

#195770

Purpose

Gateway Entry Clone of Epstein-Barr virus BNLF2b

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BNLF2b (BNLF2b Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBW313lux-hrpR

Plasmid

#61436

Purpose

pBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying BBa_F2620 (PTet-rbs34-luxR-ter))

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

F2620-rbs33-hrpR-ter

Expression

Bacterial

Promoter

plux

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-Hygro HoxB9

Plasmid

#27022

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

HoxB9 (HOXB9 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-hMcl-1(T163A)

Plasmid

#25376

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1 (MCL1 Human)

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed threonine 163 to alanine

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

Plasmid

#185687

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-2xHaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K9R

Plasmid

#84527

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

none

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
ATF4 11: 5’ATF4.uORF2.GFP

Plasmid

#21858

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF2 (uORF) of ATF4 fused to EGFP

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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R26-EGFP MMEJ donor vector

Plasmid

#137926

Purpose

R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-EGFP-bpA with micro-homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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REEP5-2Strep

Plasmid

#210350

Purpose

Expresses REEP5 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP5 (REEP5 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pUAST-SIK3 WT

Plasmid

#52972

Purpose

insect expression of SIK3 WT

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Promoter

Hsp70

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only
pDIV489

Plasmid

#177697

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pUAST-Myc-dATG1

Plasmid

#111801

Purpose

Insect Expression of dAtg1

Depositor

Jun Hee Lee

Article

Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

Insert

dAtg1 (Atg1 Fly)

Tags

Myc

Expression

Insect

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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