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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 33,758 results for: crispr

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Showing: 8421 - 8440 of 33758 results

  3. pAL-pgi_T

    Plasmid
    #74066
    Purpose
    pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR
    Depositor
    Timothy Lu
    Publication
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pgi (T)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. pEG302 22aa SunTag VP64 gRNA4 (FWA)

    Plasmid
    #120249
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    120249-plasmid-map-sequence-id-242035

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  8. pNR4A1-donor

    Plasmid
    #112295
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP
    Depositor
    Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
    Use
    CRISPR
    Mutation
    hg38:12:52058840:C>T, hg38:12:52058930:A>C,…
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112295-plasmid-map-sequence-id-228416

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  9. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid
    #181792
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181792-plasmid-map-sequence-id-358226

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  12. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid
    #99690
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Synthetic, Mouse)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available since
    Sept. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    99690-plasmid-map-sequence-id-194510

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Showing: 8421 - 8440 of 33758 results