We narrowed to 9,493 results for: pho;

1. pCAG-loxSTOPlox-LynEGFP

   Plasmid

   #133922

   Purpose

   Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombination

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   LynEGFP
   

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-mVenus-CAAX

   Plasmid

   #75153

   Purpose

   Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   mVenus-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. 14UAS PSD95:GFP 5UAS DSRedExpress

   Plasmid

   #74315

   Purpose

   Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish

   Depositor

   Martin Meyer, Stephen Smith

   Article

   Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

   Inserts

   zebrafish PSD95
   DsRedExpress
   

   Tags

   EGFP

   Mutation

   Y599H and I793S (please see depositor comment bel…

   Available Since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pVS2

   Plasmid

   #33144

   Depositor

   Michael Rosbash

   Article

   Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

   Inserts

   LacO (256 copies)
   GAL-GFP-kd200-GALpA reporter
   

   Expression

   Yeast

   Mutation

   GFP is mutated such that amino acids 64-73 (FSYGV…

   Promoter

   bidirectional GAL1-GAL10 promoter

   Available Since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

5. mCherry-C1-ORP5deltaTMD-FKBP

   Plasmid

   #220073

   Purpose

   expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL5deltaTMD (OSBPL5 Human)
   

   Expression

   Mammalian

   Mutation

   deltaTMD

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTF102

   Plasmid

   #134774

   Purpose

   A binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.

   Depositor

   Kan Wang

   Article

   Frame et al Plant Physiol. 2002 May;129(1):13-22. do

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACAGW-H2B-PAGFP-AAV

   Plasmid

   #33000

   Depositor

   Massimo Scanziani

   Article

   Lien et al Front Neural Circuits. 2011;5:16. Epub 2

   Insert

   H2B-PAGFP (H2BC21 Human, Aequorea victoria)
   

   Use

   AAV; Adeno associated virus

   Tags

   PAGFP

   Expression

   Mammalian

   Available Since

   Dec. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. PAmCherry1-C1-P4Mx2

   Plasmid

   #202681

   Purpose

   Photoactivatable lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   PAmCherry1: L. pneumophila SidM(546-647):SidM(546-647) (sidM Legionella pneumophila)
   

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PAmCherry1-C1-P4C

   Plasmid

   #202680

   Purpose

   Photoactivatable lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   PAmCherry1: L. pneumophila SidC (608-773) (sidC Legionella pneumophila)
   

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTfR-PAmKate

    Plasmid

    #32692

    Depositor

    Vladislav Verkhusha

    Article

    Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

    Insert

    PAmKate
    

    Tags

    transferrin receptor (TfR)

    Expression

    Mammalian

    Mutation

    F84W/S148N/S165G compared to mKate (but numbering…

    Promoter

    CMV

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-ChrimsonR-mRuby2-ST

    Plasmid

    #105447

    Purpose

    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Insert

    ChrimsonR-mRuby2-ST
    

    Tags

    mRuby2 and soma targeting motif

    Expression

    Mammalian

    Mutation

    K176R in Chrimson

    Promoter

    pCAGGS

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMART-HCKan-yibDp-VHH-VCAM1

    Plasmid

    #202471

    Purpose

    Expresses the nanobody VCAM1 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VCAM1
    

    Tags

    6X-His

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2

    Plasmid

    #191338

    Purpose

    Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    TUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
    

    Expression

    Mammalian

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbE5a-Opto-Cre-Vvd

    Plasmid

    #134404

    Purpose

    Light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

    Depositor

    Mary Dunlop

    Article

    Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

    Insert

    Opto-Cre-Vvd
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Jan. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSF-CjPglB-LpxE

    Plasmid

    #128389

    Purpose

    Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Inserts

    PglB oligosaccharylktransferase
    LpxE phosphatase
    

    Tags

    Flag tag

    Expression

    Bacterial

    Promoter

    Ptac and pBAD

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMVTO-hIL2

    Plasmid

    #233387

    Purpose

    secreted human IL-2

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    IL-2 (IL2 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Avr2-GFP pZK538

    Plasmid

    #118011

    Purpose

    expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .

    Depositor

    FLW Takken

    Article

    et al Bio-protocol 8(24): e3114.

    Inserts

    dspAvr2
    mCherry
    

    Tags

    EGFP and HDEL

    Expression

    Bacterial and Plant

    Promoter

    35S

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. mPA-GFP-Tubulin-C-18

    Plasmid

    #57160

    Purpose

    Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Article

    Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    mPA-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. His6-TEV-mCerulean3-pBluescript

    Plasmid

    #166757

    Purpose

    For purification of mCerulean3 fluorophore

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    mCerulean3
    

    Tags

    6x Histidine tag, and TEV protease site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-CoChR-mRuby2-ST

    Plasmid

    #109131

    Purpose

    Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    CoChR-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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